This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores. This improved set offers the high brightness that has made Semrock famous, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications that is associated with shorter-wavelength yellow and green fluorescent markers, like yellow fluorescent protein (YFP), green fluorescent protein (GFP), and FITC. Note that the narrow excitation filter efficiently captures the 546 nm emission line of mercury or metal-halide-mercury arc lamps.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,623.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-593/40-25
FL-004557 / 0
- Tavg > 93% 573 – 613 nm
- Center Wavelength of 593 nm and GMBW of 40 nm
- FWHM of 45.7 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-530 | 537 | 560 |
|
|
| DyLight 549 | 553 | 569 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| Gold 550 | 551 | 564 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 555 | 550 | 566 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
|
| mCherry | 587 | 610 |
|
|
| Merocyanine 540 | 559 | 579 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| mKO | 549 | 559 |
|
|
| mOrange | 546 | 562 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Orange 552 | 552 | 574 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| POPO-3 | 533 | 574 |
|
|
| PO-PRO-3 | 539 | 570 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SpectrumGold | 530 | 556 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboRFP | 553 | 573 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-593/40-25
FL-004557 / 0
- Tavg > 93% 573 – 613 nm
- Center Wavelength of 593 nm and GMBW of 40 nm
- FWHM of 45.7 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
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LL01-405-12.5
FL-007943