This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores. This improved set offers the high brightness that has made Semrock famous, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications that is associated with shorter-wavelength yellow and green fluorescent markers, like yellow fluorescent protein (YFP), green fluorescent protein (GFP), and FITC. Note that the narrow excitation filter efficiently captures the 546 nm emission line of mercury or metal-halide-mercury arc lamps.
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||21.8 mm||5.0 mm|
|Emitter (Diameter)||23.3 mm||5.0 mm|
|Dichroic (L × W)||22.0 mm × 29.0 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|