This filter set is ideal for use with TRITC and other similar fluorophores. When viewing TRITC visually, it has the "reddish" appearance with which many experienced microscopists are familiar. This hard-coated filter set is the best value on the market, delivering high brightness and contrast.
Note: Historical limitations in filter technology led to the widespread use of TRITC emission filters that are overly broad and red-shifted, causing the TRITC fluorescence to appear red. This BrightLine Basic filter set delivers that familiar "reddish" appearance. In contrast, Semrock's highest-performance BrightLine TRITC-B filter set is optimized for the best signal and noise performance, and since the TRITC emission band peaks in the orange, the fluorescence correctly appears orange – not red.
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||5.0 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?|