BrightLine Basic single-band filter set, optimized for TRITC and other like fluorophores

Semrock Part Number: TRITC-A-Basic-OFF

IDEX Part Number: S-000572  /

Semrock

This filter set is ideal for use with TRITC and other similar fluorophores. When viewing TRITC visually, it has the "reddish" appearance with which many experienced microscopists are familiar. This hard-coated filter set is the best value on the market, delivering high brightness and contrast.

Note: Historical limitations in filter technology led to the widespread use of TRITC emission filters that are overly broad and red-shifted, causing the TRITC fluorescence to appear red. This BrightLine Basic filter set delivers that familiar "reddish" appearance. In contrast, Semrock's highest-performance BrightLine TRITC-B filter set is optimized for the best signal and noise performance, and since the TRITC emission band peaks in the orange, the fluorescence correctly appears orange – not red.

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FF01-542/20-25

FL-004453 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 533 – 552.6 nm
  • Center Wavelength of 542.8 nm and GMBW of 19.6 nm
  • FWHM of 24.9 nm

FF01-620/52-25

FL-004561 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 594.0 – 646.9 nm
  • Center Wavelength of 620 nm and GMBW of 51.9 nm
  • FWHM of 58.2 nm

FF570-Di01-25x36

FL-006974 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 570 nm
  • Ravg > 90% 525 – 556 nm
  • Tavg > 90% 580 – 650 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 546 556 572
Alexa Fluor® 568 579 603
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 550 553 576
ATTO 565 563 592
BOBO™-3 570 605
BODIPY TR-X phallacidin 590 621
Calcium Orange 589 609
CellTracker Red CMTPX 586 614
Cy3.5™ 578 591
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
KFP-Red 575 599
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
MitoTracker™ Orange 551 575
mKate2 588 633
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
Tetramethylrhodamine dextran 554 582
Texas Red DHPE 584 608
TRITC (Tetramethylrhodamine) 552 578
TRITC (Tetramethylrhodamine) - "reddish" appearance 545 620
TurboRFP 553 573
X-Rhod-1 Indicator 580 601

Related Products

FF01-542/20-25

FL-004453 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 533 – 552.6 nm
  • Center Wavelength of 542.8 nm and GMBW of 19.6 nm
  • FWHM of 24.9 nm

FF01-620/52-25

FL-004561 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 594.0 – 646.9 nm
  • Center Wavelength of 620 nm and GMBW of 51.9 nm
  • FWHM of 58.2 nm

FF570-Di01-25x36

FL-006974 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 570 nm
  • Ravg > 90% 525 – 556 nm
  • Tavg > 90% 580 – 650 nm
  •  Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.