This filter set is ideal for use with TRITC and other similar fluorophores. When viewing TRITC visually, it has the "reddish" appearance with which many experienced microscopists are familiar. This hard-coated filter set is the best value on the market, delivering high brightness and contrast.
Note: Historical limitations in filter technology led to the widespread use of TRITC emission filters that are overly broad and red-shifted, causing the TRITC fluorescence to appear red. This BrightLine Basic filter set delivers that familiar "reddish" appearance. In contrast, Semrock's highest-performance BrightLine TRITC-B filter set is optimized for the best signal and noise performance, and since the TRITC emission band peaks in the orange, the fluorescence correctly appears orange – not red.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,374.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-542/20-25
FL-004453 / 0
- Tavg > 90% 533 – 552.6 nm
- Center Wavelength of 542.8 nm and GMBW of 19.6 nm
- FWHM of 24.9 nm
FF01-620/52-25
FL-004561 / 0
- Tavg > 90% 594.0 – 646.9 nm
- Center Wavelength of 620 nm and GMBW of 51.9 nm
- FWHM of 58.2 nm
FF570-Di01-25x36
FL-006974 / 0
- Dichroic Beamsplitter with edge at 570 nm
- Ravg > 90% 525 – 556 nm
- Tavg > 90% 580 – 650 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
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| Cy3.5™ | 578 | 591 |
|
|
| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| KFP-Red | 575 | 599 |
|
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| LysoTracker Red | 573 | 592 |
|
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| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| mCherry | 587 | 610 |
|
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| MitoTracker™ Orange | 551 | 575 |
|
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| mKate2 | 588 | 633 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
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| Orange 552 | 552 | 574 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| Resorufin | 571 | 584 |
|
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| Rhod-2 | 553 | 577 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| SpectrumOrange | 554 | 587 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| Texas Red DHPE | 584 | 608 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TRITC (Tetramethylrhodamine) - "reddish" appearance | 545 | 620 |
|
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| TurboRFP | 553 | 573 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-542/20-25
FL-004453 / 0
- Tavg > 90% 533 – 552.6 nm
- Center Wavelength of 542.8 nm and GMBW of 19.6 nm
- FWHM of 24.9 nm
FF01-620/52-25
FL-004561 / 0
- Tavg > 90% 594.0 – 646.9 nm
- Center Wavelength of 620 nm and GMBW of 51.9 nm
- FWHM of 58.2 nm
FF570-Di01-25x36
FL-006974 / 0
- Dichroic Beamsplitter with edge at 570 nm
- Ravg > 90% 525 – 556 nm
- Tavg > 90% 580 – 650 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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