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BrightLine® single-band filter set, optimized for Spectrum Orange and other like fluorophores

Semrock Part Number: SpOr-B-NTE

IDEX Part Number: S-000554  /

Semrock

This filter set is optimized to be the highest signal-to-noise ratio (contrast) available for measuring Spectrum Orange. This set is the unparalleled choice for most situations, especially those involving multi-color FISH measurements. For specialized applications where brightness trumps contrast, use our TRITC-B set, which will provide a significant increase in brightness.

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,469.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-586/20-25x3.5

FL-004495 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 576 – 596 nm
  • Center Wavelength of 586 nm and GMBW of 20 nm
  • FWHM of 25.9 nm

FF01-543/22-25

FL-004717 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 532 – 554 nm
  • Center Wavelength of 543 nm and GMBW of 22 nm
  • FWHM of 27.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Amplex UltraRed peroxidation product (pH 7.5) 568 581
ATTO 532 501 545
ATTO 550 553 576
ATTO 565 563 592
BOBO™-3 570 605
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcium Orange 589 609
Cy3.5™ 578 591
Cy3™ 554 566
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-530 537 560
DyLight 549 553 569
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Orange fluorescent microspheres 540 559
Gold 550 551 564
HiLyte Fluor™ 555 550 566
KFP-Red 575 599
LOLO-1 568 580
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
mKO 549 559
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
POPO-3 533 574
PO-PRO-3 539 570
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
R-phycoerythrin (PE) 565 576
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
SpectrumGold 530 556
SpectrumOrange 554 587
SYTO 82 540 559
SYTOX Orange 547 570
Tetramethylrhodamine dextran 554 582
TRITC (Tetramethylrhodamine) 552 578
TurboRFP 553 573
VIC 530 550

Related Products

FF01-586/20-25x3.5

FL-004495 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 576 – 596 nm
  • Center Wavelength of 586 nm and GMBW of 20 nm
  • FWHM of 25.9 nm

FF01-543/22-25

FL-004717 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 532 – 554 nm
  • Center Wavelength of 543 nm and GMBW of 22 nm
  • FWHM of 27.1 nm

FF562-Di03-25x36

FL-007122 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 562 nm
  • Ravg > 98% 350 – 554.5 nm
  • Tavg > 93% 569.5 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.