This filter set is optimized to be the highest signal-to-noise ratio (contrast) available for measuring Spectrum Orange. This set is the unparalleled choice for most situations, especially those involving multi-color FISH measurements. For specialized applications where brightness trumps contrast, use our TRITC-B set, which will provide a significant increase in brightness.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,593.78 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-586/20-25x3.5
FL-004495 / 0
- Tavg > 93% 576 – 596 nm
- Center Wavelength of 586 nm and GMBW of 20 nm
- FWHM of 25.9 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-530 | 537 | 560 |
|
|
| DyLight 549 | 553 | 569 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
|
| Gold 550 | 551 | 564 |
|
|
| HiLyte Fluor™ 555 | 550 | 566 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
|
| Merocyanine 540 | 559 | 579 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| mKO | 549 | 559 |
|
|
| mOrange | 546 | 562 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mRFP | 555 | 583 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Orange 552 | 552 | 574 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| POPO-3 | 533 | 574 |
|
|
| PO-PRO-3 | 539 | 570 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| R-phycoerythrin (PE) | 565 | 576 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SpectrumGold | 530 | 556 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboRFP | 553 | 573 |
|
|
| VIC | 530 | 550 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-586/20-25x3.5
FL-004495 / 0
- Tavg > 93% 576 – 596 nm
- Center Wavelength of 586 nm and GMBW of 20 nm
- FWHM of 25.9 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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Di03-R532-t3-25x36
FL-006803