This filter set is optimized to be the highest signal-to-noise ratio (contrast) available for measuring Spectrum Orange. This set is the unparalleled choice for most situations, especially those involving multi-color FISH measurements. For specialized applications where brightness trumps contrast, use our TRITC-B set, which will provide a significant increase in brightness.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,169.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
FF01-543/22-25FL-004717
Each
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FL-004717 |
|
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FL-003876 |
|
|
|
FL-007169 |
total selected
Clear selection
Select all 3 products from all pages.
FF01-586/20-25x3.5
FL-004495 / 0
- Tavg > 93% 576 – 596 nm
- Center Wavelength of 586 nm and GMBW of 20 nm
- FWHM of 25.9 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| Alexa Fluor® 532 | 534 | 553 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 555 | 553 | 565 |
|
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| ATTO 532 | 501 | 545 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
|
| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| Cy3.5™ | 578 | 591 |
|
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| Cy3™ | 554 | 566 |
|
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| Dendra2 (Red) | 554 | 569 |
|
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| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
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| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-530 | 537 | 560 |
|
|
| DyLight 549 | 553 | 569 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
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| Gold 550 | 551 | 564 |
|
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| HiLyte Fluor™ 555 | 550 | 566 |
|
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| KFP-Red | 575 | 599 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| Magnesium Orange | 550 | 574 |
|
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| mApple | 566 | 594 |
|
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| Merocyanine 540 | 559 | 579 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
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| mKO | 549 | 559 |
|
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| mOrange | 546 | 562 |
|
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| mOrange2 | 551 | 565 |
|
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| mRFP | 555 | 583 |
|
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| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| Nile red-triglyceride | 510 | 583 |
|
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| OFP | 547 | 567 |
|
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| Orange 552 | 552 | 574 |
|
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| POPO-3 | 533 | 574 |
|
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| PO-PRO-3 | 539 | 570 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
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| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SpectrumGold | 530 | 556 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTOX Orange | 547 | 570 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboRFP | 553 | 573 |
|
|
| VIC | 530 | 550 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-586/20-25x3.5
FL-004495 / 0
- Tavg > 93% 576 – 596 nm
- Center Wavelength of 586 nm and GMBW of 20 nm
- FWHM of 25.9 nm
FF01-543/22-25
FL-004717 / 0
- Tavg > 93% 532 – 554 nm
- Center Wavelength of 543 nm and GMBW of 22 nm
- FWHM of 27.1 nm
FF562-Di03-25x36
FL-007122 / 0
- Dichroic Beamsplitter with edge at 562 nm
- Ravg > 98% 350 – 554.5 nm
- Tavg > 93% 569.5 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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