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BrightLine® single-band filter set, optimized for Spectrum Green and other like fluorophores

Semrock Part Number: SpGr-B-NQF

IDEX Part Number: S-000544  /

Semrock

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Green.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,489.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
Acridine Orange (+DNA) 502 526
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
ATTO 488 500 525
ATTO 495 495 527
AzamiGreen 494 505
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Venus 516 528
Vybrant DyeCycle Green 506 534
YFP (yellow GFP) 513 530
YO-PRO-1 491 506
YOYO-1 491 508

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2 mm dichroic)

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