This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Green.
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 514 | 518 | 543 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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AzamiGreen | 494 | 505 |
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BCECF (pH 5.5) | 481 | 518 |
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BCECF (pH 9.0) | 502 | 528 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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Calcein | 494 | 514 |
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Calcium Green-1 | 507 | 529 |
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CFP2 | 490 | 510 |
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Cy2â„¢ | 492 | 507 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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Dendra2 (Green) | 491 | 507 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DY-505-Phalloidin | 503 | 530 |
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Emerald | 491 | 511 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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GFP (EGFP) | 489 | 511 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HiLyte Fluorâ„¢ 488 | 497 | 526 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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mHoneyDew | 478 | 562 |
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MitoTrackerâ„¢ Green | 490 | 512 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Oregon Greenâ„¢ 488 | 498 | 526 |
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Oregon Greenâ„¢ 514 | 513 | 532 |
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PicoGreen® | 502 | 522 |
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PKH67 | 488 | 500 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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ratiometric pHluorin pH5 | 500 | 509 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine 123 | 507 | 529 |
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Rhodamine Green | 497 | 523 |
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Rhodol Green | 497 | 524 |
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Sodium Green | 507 | 532 |
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SpectrumGreen | 497 | 538 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYTO 11 | 506 | 525 |
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SYTO 13 | 448 | 506 |
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SYTO 16 | 489 | 520 |
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SYTO 9 | 483 | 500 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TurboGFP | 482 | 503 |
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Venus | 516 | 528 |
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Vybrant DyeCycle Green | 506 | 534 |
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YFP (yellow GFP) | 513 | 530 |
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YO-PRO-1 | 491 | 506 |
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YOYO-1 | 491 | 508 |
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |