This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Gold.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,539.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-534/20-25
FL-004420 / 0
- Tavg > 93% 524 – 544 nm
- Center Wavelength of 534 nm and GMBW of 20 nm
- FWHM of 25.6 nm
FF01-572/28-25
FL-004422 / 0
- Tavg > 93% 558 – 586 nm
- Center Wavelength of 572 nm and GMBW of 28 nm
- FWHM of 34.1 nm
FF552-Di02-25x36
FL-007121 / 0
- Dichroic Beamsplitter with edge at 552 nm
- Ravg > 98% 350 – 544 nm
- Tavg > 93% 558 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 6-JOE | 520 | 548 |
|
|
| Alexa Fluor® 514 | 518 | 543 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| Alexa Fluor® 546 | 556 | 572 |
|
|
| Alexa Fluor® 555 | 553 | 565 |
|
|
| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
|
| ATTO 520 | 516 | 538 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| ATTO 550 | 553 | 576 |
|
|
| BODIPY R6G | 528 | 547 |
|
|
| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
|
| Calcium Orange | 589 | 609 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| Cy3™ | 554 | 566 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| DiI | 551 | 569 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-530 | 537 | 560 |
|
|
| DyLight 549 | 553 | 569 |
|
|
| Eosin | 525 | 546 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| FluoSpheres Orange fluorescent microspheres | 540 | 559 |
|
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| Gold 525 | 521 | 551 |
|
|
| Gold 550 | 551 | 564 |
|
|
| HiLyte Fluor™ 555 | 550 | 566 |
|
|
| JO-JO-1, JO-PRO-1 | 530 | 544 |
|
|
| LOLO-1 | 568 | 580 |
|
|
| Magnesium Orange | 550 | 574 |
|
|
| mApple | 566 | 594 |
|
|
| Merocyanine 540 | 559 | 579 |
|
|
| MitoTracker™ Orange | 551 | 575 |
|
|
| mKO | 549 | 559 |
|
|
| mOrange | 546 | 562 |
|
|
| mOrange2 | 551 | 565 |
|
|
| mRFP | 555 | 583 |
|
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| mStrawberry | 574 | 596 |
|
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| mTangerine | 568 | 585 |
|
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| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
|
| Orange 552 | 552 | 574 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| POPO-3 | 533 | 574 |
|
|
| PO-PRO-3 | 539 | 570 |
|
|
| Pro-Q Diamond | 556 | 583 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Resorufin | 571 | 584 |
|
|
| Rhod-2 | 553 | 577 |
|
|
| Rhodamine phalloidin | 558 | 575 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SpectrumGold | 530 | 556 |
|
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| SpectrumOrange | 554 | 587 |
|
|
| SYTO 82 | 540 | 559 |
|
|
| SYTOX Orange | 547 | 570 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
|
| TurboRFP | 553 | 573 |
|
|
| TurboYFP | 525 | 538 |
|
|
| VIC | 530 | 550 |
|
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| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| ZsYellow1 | 529 | 539 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-534/20-25
FL-004420 / 0
- Tavg > 93% 524 – 544 nm
- Center Wavelength of 534 nm and GMBW of 20 nm
- FWHM of 25.6 nm
FF01-572/28-25
FL-004422 / 0
- Tavg > 93% 558 – 586 nm
- Center Wavelength of 572 nm and GMBW of 28 nm
- FWHM of 34.1 nm
FF552-Di02-25x36
FL-007121 / 0
- Dichroic Beamsplitter with edge at 552 nm
- Ravg > 98% 350 – 544 nm
- Tavg > 93% 558 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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