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BrightLine® single-band filter set, optimized for Spectrum Gold and other like fluorophores

Semrock Part Number: SpGold-B-NQF-ZERO

IDEX Part Number: S-001943  /

Semrock
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
  • Price Not Available.
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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,598.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Amplex UltraRed peroxidation product (pH 7.5) 568 581
ATTO 520 516 538
ATTO 532 501 545
ATTO 550 553 576
BODIPY R6G 528 547
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
Calcium Orange 589 609
Cy3.5™ 578 591
Cy3™ 554 566
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-530 537 560
DyLight 549 553 569
Eosin 525 546
Ethidium bromide 518 603
FluoSpheres Orange fluorescent microspheres 540 559
Gold 525 521 551
Gold 550 551 564
HiLyte Fluor™ 555 550 566
JO-JO-1, JO-PRO-1 530 544
LOLO-1 568 580
Magnesium Orange 550 574
mApple 566 594
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
mKO 549 559
mOrange 546 562
mOrange2 551 565
mRFP 555 583
mStrawberry 574 596
mTangerine 568 585
Nile red-triglyceride 510 583
OFP 547 567
Orange 552 552 574
pHrodo™, succinimidyl ester 560 587
POPO-3 533 574
PO-PRO-3 539 570
Pro-Q Diamond 556 583
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
R-phycoerythrin (PE) 565 576
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
SpectrumGold 530 556
SpectrumOrange 554 587
SYTO 82 540 559
SYTOX Orange 547 570
Tetramethylrhodamine dextran 554 582
TRITC (Tetramethylrhodamine) 552 578
TurboRFP 553 573
TurboYFP 525 538
VIC 530 550
Vybrant DyeCycle Orange 519 563
ZsYellow1 529 539

NQF Holder/Cube

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)

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