A special long-pass emission filter in this filter set allows simultaneous viewing by eye or with a color camera of samples that are multi-labeled with quantum dot nanocrystals. It takes advantage of the unique fact that all quantum dots share a common UV/blue excitation spectrum while simultaneously exhibiting narrow and specific visible emission spectra. The highly transmitting, deep-blue exciter efficiently excites all quantum dots with no UV light to damage samples or generate undesirable background, while the high and uniform transmission of the long-pass emission filter provides exceptional and balanced brightness. And with the "no burn-out" performance shared by all BrightLine filters, the permanent performance of this set will outlast even your quantum dots!
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,508.94 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
FF01-500/LP-25
FL-009066 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Orange (+RNA) | 460 | 650 |
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| Alexa Fluor® 430 | 431 | 541 |
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| ATTO 465 | 453 | 507 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BD Horizon V500 | 410 | 500 |
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| Cascade Yellow™ | 399 | 549 |
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| CellTracker Violet BMQC+GSH | 406 | 526 |
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| CFP (ECFP) | 433 | 475 |
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| ChlorophyllA | 480 | 650 |
|
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| Di-8-ANEPPS | 467 | 631 |
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| DiA | 457 | 586 |
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| DsRed-Express | 556 | 584 |
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| FM 1-43 | 473 | 579 |
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| Fura Red™ (high pH) | 572 | 657 |
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| Lucifer yellow | 428 | 544 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| mHoneyDew | 478 | 562 |
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| NBD-X (MeOH) | 467 | 538 |
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| Pacific Orange | 400 | 551 |
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| PerCP | 490 | 677 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 565 Nanocrystals | 300 | 564 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Qdot® 800 Nanocrystals | 300 | 792 |
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| SYPRO Ruby protein gel stain | 469 | 631 |
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| ThiolTracker Violet+GSH | 404 | 526 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
FF01-500/LP-25
FL-009066 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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BLP01-473R-25
FL-008548