A special long-pass emission filter in this filter set allows simultaneous viewing by eye or with a color camera of samples that are multi-labeled with quantum dot nanocrystals. It takes advantage of the unique fact that all quantum dots share a common UV/blue excitation spectrum while simultaneously exhibiting narrow and specific visible emission spectra. The highly transmitting, deep-blue exciter efficiently excites all quantum dots with no UV light to damage samples or generate undesirable background, while the high and uniform transmission of the long-pass emission filter provides exceptional and balanced brightness. And with the "no burn-out" performance shared by all BrightLine filters, the permanent performance of this set will outlast even your quantum dots!
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This set is mounted in a Leica Microsystems DMi8 (“P-Cube”) Fluorescence Filter Holder, which is compatible with the DMi8 Leica microscope.
|Filter Role||Filter Size||Maximum Thickness|
|Exciter (Diameter)||25.0 mm||5.0 mm|
|Emitter (Diameter)||25.0 mm||3.5 mm|
|Dichroic (L × W)||25.2 mm × 35.6 mm||1.1 mm|
|Cleaning Optical Filters||Instructions for cleaning hard coated optical filters|
|Filter Reliability||Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters|
|Working with Optical Density||Tables to describe the relationship between OD and transmission with tips for quickly calculating OD|
|What is Pixel Shift, and how does the Semrock ZERO™ option help?||Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.|