A special long-pass emission filter in this filter set allows simultaneous viewing by eye or with a color camera of samples that are multi-labeled with quantum dot nanocrystals. It takes advantage of the unique fact that all quantum dots share a common UV/blue excitation spectrum while simultaneously exhibiting narrow and specific visible emission spectra. The highly transmitting, deep-blue exciter efficiently excites all quantum dots with no UV light to damage samples or generate undesirable background, while the high and uniform transmission of the long-pass emission filter provides exceptional and balanced brightness. And with the "no burn-out" performance shared by all BrightLine filters, the permanent performance of this set will outlast even your quantum dots!
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,474.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
FF01-500/LP-25
FL-009066 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Orange (+RNA) | 460 | 650 |
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| Alexa Fluor® 430 | 431 | 541 |
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| ATTO 465 | 453 | 507 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BD Horizon V500 | 410 | 500 |
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| Cascade Yellow™ | 399 | 549 |
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| CellTracker Violet BMQC+GSH | 406 | 526 |
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| CFP (ECFP) | 433 | 475 |
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| ChlorophyllA | 480 | 650 |
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| Di-8-ANEPPS | 467 | 631 |
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| DiA | 457 | 586 |
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| DsRed-Express | 556 | 584 |
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| FM 1-43 | 473 | 579 |
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| Fura Red™ (high pH) | 572 | 657 |
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| Lucifer yellow | 428 | 544 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| mHoneyDew | 478 | 562 |
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| NBD-X (MeOH) | 467 | 538 |
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| Pacific Orange | 400 | 551 |
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| PerCP | 490 | 677 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| Qdot® 565 Nanocrystals | 300 | 564 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Qdot® 800 Nanocrystals | 300 | 792 |
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| SYPRO Ruby protein gel stain | 469 | 631 |
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| ThiolTracker Violet+GSH | 404 | 526 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
FF01-500/LP-25
FL-009066 / 0
- Filter with 50% edge at 515 nm
- Tavg > 90% 519 – 700 nm
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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