BrightLine® single-band filter set, optimized for Qdot 655 Nanocrystals and other like fluorophores

Semrock Part Number: QD655-C-OFF-ZERO

IDEX Part Number: S-001917  /

Semrock

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 655 emission with minimal crosstalk from neighboring Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

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  • Price Not Available.
  • 1,698.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-655/15-25

FL-004262 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 647.5 – 662.5 nm
  • Center Wavelength of 655 nm and GMBW of 15 nm
  • FWHM of 23.7 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Orange (+RNA) 460 650
ChlorophyllA 480 650
Di-8-ANEPPS 467 631
Fura Red™ (high pH) 572 657
PerCP 490 677
Qdot® 655 Nanocrystals 300 654
SYPRO Ruby protein gel stain 469 631

Related Products

FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-655/15-25

FL-004262 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 647.5 – 662.5 nm
  • Center Wavelength of 655 nm and GMBW of 15 nm
  • FWHM of 23.7 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.