BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Semrock Part Number: QD525-C-ZHE

IDEX Part Number: S-001888  /

Semrock

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

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  • 1,454.00 USD Each
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FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-525/15-25

FL-004162 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 517.5 – 532.5 nm
  • Center Wavelength of 525 nm and GMBW of 15 nm
  • FWHM of 18.4 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
AmCyan1 458 489
ATTO 425 436 484
ATTO 465 453 507
BCECF (pH 5.5) 481 518
BD Horizon V500 410 500
BOBO™-1 461 484
Cascade Yellow™ 399 549
CellTracker Violet BMQC+GSH 406 526
Cerulean 434 473
CFP (ECFP) 433 475
DEAC 432 472
ECFP 435 475
evoglow-Bs1 449 496
GFP (EGFP) 489 511
Lucifer yellow 428 544
LysoSensor Green 448 502
mBBr+GSH 394 490
mHoneyDew 478 562
NBD-X (MeOH) 467 538
Pacific Orange 400 551
Qdot® 525 Nanocrystals 300 525
sgGFP™ (super glow GFP) 472 506
SpectrumAqua 434 481
SYTO 45 451 485
TagCFP 458 480
ThiolTracker Violet+GSH 404 526
wtGFP (wild type GFP, non-UV excitation) 474 509

Related Products

FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-525/15-25

FL-004162 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 517.5 – 532.5 nm
  • Center Wavelength of 525 nm and GMBW of 15 nm
  • FWHM of 18.4 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help?
Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.