BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Semrock Part Number: QD525-C-OMF

IDEX Part Number: S-001886  /

Semrock

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

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  • 1,614.00 USD Each
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FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-525/15-25

FL-004162 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 517.5 – 532.5 nm
  • Center Wavelength of 525 nm and GMBW of 15 nm
  • FWHM of 18.4 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
AmCyan1 458 489
ATTO 425 436 484
ATTO 465 453 507
BCECF (pH 5.5) 481 518
BD Horizon V500 410 500
BOBO™-1 461 484
Cascade Yellow™ 399 549
CellTracker Violet BMQC+GSH 406 526
Cerulean 434 473
CFP (ECFP) 433 475
DEAC 432 472
ECFP 435 475
evoglow-Bs1 449 496
GFP (EGFP) 489 511
Lucifer yellow 428 544
LysoSensor Green 448 502
mBBr+GSH 394 490
mHoneyDew 478 562
NBD-X (MeOH) 467 538
Pacific Orange 400 551
Qdot® 525 Nanocrystals 300 525
sgGFP™ (super glow GFP) 472 506
SpectrumAqua 434 481
SYTO 45 451 485
TagCFP 458 480
ThiolTracker Violet+GSH 404 526
wtGFP (wild type GFP, non-UV excitation) 474 509

Related Products

FF02-435/40-25

FL-003733 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FF01-525/15-25

FL-004162 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 517.5 – 532.5 nm
  • Center Wavelength of 525 nm and GMBW of 15 nm
  • FWHM of 18.4 nm

FF510-Di02-25x36

FL-007118 / 0

Out of Stock Backordered In Stock
  • Dichroic Beamsplitter with edge at 510 nm
  • Ravg > 98% 327 – 488 nm
  • Tavg > 93% 515 – 950 nm
  • Flatness / RWE: Standard Epi-fluorescence

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm