This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,564.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF01-525/15-25
FL-004162 / 0
- Tavg > 90% 517.5 – 532.5 nm
- Center Wavelength of 525 nm and GMBW of 15 nm
- FWHM of 18.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 430 | 431 | 541 |
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| AmCyan1 | 458 | 489 |
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| ATTO 425 | 436 | 484 |
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| ATTO 465 | 453 | 507 |
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BD Horizon V500 | 410 | 500 |
|
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| BOBO™-1 | 461 | 484 |
|
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| Cascade Yellow™ | 399 | 549 |
|
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| CellTracker Violet BMQC+GSH | 406 | 526 |
|
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| Cerulean | 434 | 473 |
|
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| CFP (ECFP) | 433 | 475 |
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| DEAC | 432 | 472 |
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| ECFP | 435 | 475 |
|
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| evoglow-Bs1 | 449 | 496 |
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| GFP (EGFP) | 489 | 511 |
|
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| Lucifer yellow | 428 | 544 |
|
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| LysoSensor Green | 448 | 502 |
|
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| mBBr+GSH | 394 | 490 |
|
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| mHoneyDew | 478 | 562 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| Pacific Orange | 400 | 551 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SpectrumAqua | 434 | 481 |
|
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| SYTO 45 | 451 | 485 |
|
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| TagCFP | 458 | 480 |
|
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| ThiolTracker Violet+GSH | 404 | 526 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF01-525/15-25
FL-004162 / 0
- Tavg > 90% 517.5 – 532.5 nm
- Center Wavelength of 525 nm and GMBW of 15 nm
- FWHM of 18.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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