This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,673.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF01-525/15-25
FL-004162 / 0
- Tavg > 90% 517.5 – 532.5 nm
- Center Wavelength of 525 nm and GMBW of 15 nm
- FWHM of 18.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 430 | 431 | 541 |
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| AmCyan1 | 458 | 489 |
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| ATTO 425 | 436 | 484 |
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| ATTO 465 | 453 | 507 |
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BD Horizon V500 | 410 | 500 |
|
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| BOBO™-1 | 461 | 484 |
|
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| Cascade Yellow™ | 399 | 549 |
|
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| CellTracker Violet BMQC+GSH | 406 | 526 |
|
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| Cerulean | 434 | 473 |
|
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| CFP (ECFP) | 433 | 475 |
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| DEAC | 432 | 472 |
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| ECFP | 435 | 475 |
|
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| evoglow-Bs1 | 449 | 496 |
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| GFP (EGFP) | 489 | 511 |
|
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| Lucifer yellow | 428 | 544 |
|
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| LysoSensor Green | 448 | 502 |
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| mBBr+GSH | 394 | 490 |
|
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| mHoneyDew | 478 | 562 |
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| NBD-X (MeOH) | 467 | 538 |
|
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| Pacific Orange | 400 | 551 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| SpectrumAqua | 434 | 481 |
|
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| SYTO 45 | 451 | 485 |
|
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| TagCFP | 458 | 480 |
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| ThiolTracker Violet+GSH | 404 | 526 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-435/40-25
FL-003733 / 0
- Tavg > 90% 415 – 455 nm
- Center Wavelength of 435 nm and GMBW of 40 nm
- FWHM of 48.4 nm
FF01-525/15-25
FL-004162 / 0
- Tavg > 90% 517.5 – 532.5 nm
- Center Wavelength of 525 nm and GMBW of 15 nm
- FWHM of 18.4 nm
FF510-Di02-25x36
FL-007118 / 0
- Dichroic Beamsplitter with edge at 510 nm
- Ravg > 98% 327 – 488 nm
- Tavg > 93% 515 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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