BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Semrock Part Number: QD525-C-000-ZERO

IDEX Part Number: S-003229  /

Semrock

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,264.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-525/15

FL-001589

Each

FL-001589

FF02-435/40-25

FL-003733

Each
  • Tavg > 90% 415 – 455 nm
  • Center Wavelength of 435 nm and GMBW of 40 nm
  • FWHM of 48.4 nm

FL-003733

FF510-Di02

FL-006584

Each

FL-006584

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
Alexa Fluor® 430 431 541
AmCyan1 458 489
ATTO 425 436 484
ATTO 465 453 507
BCECF (pH 5.5) 481 518
BD Horizon V500 410 500
BOBO™-1 461 484
Cascade Yellow™ 399 549
CellTracker Violet BMQC+GSH 406 526
Cerulean 434 473
CFP (ECFP) 433 475
DEAC 432 472
ECFP 435 475
evoglow-Bs1 449 496
GFP (EGFP) 489 511
Lucifer yellow 428 544
LysoSensor Green 448 502
mBBr+GSH 394 490
mHoneyDew 478 562
NBD-X (MeOH) 467 538
Pacific Orange 400 551
Qdot® 525 Nanocrystals 300 525
sgGFP™ (super glow GFP) 472 506
SpectrumAqua 434 481
SYTO 45 451 485
TagCFP 458 480
ThiolTracker Violet+GSH 404 526
wtGFP (wild type GFP, non-UV excitation) 474 509

Fluorophore