This filter set is ideal for use with the monomeric fluorescent protein mCherry. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noi
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FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-562/40-25
FL-004456 / 0
- Tavg > 93% 542 – 582 nm
- Center Wavelength of 562 nm and GMBW of 40 nm
- FWHM of 45.5 nm
FF593-Di03-25x36
FL-007026 / 0
- Dichroic Beamsplitter with edge at 593 nm
- Ravg > 98% 350 – 585 nm
- Tavg > 93% 601 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| AsRed 2 | 576 | 592 |
|
|
| ATTO 565 | 563 | 592 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| BOBO™-3 | 570 | 605 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| Cy3.5™ | 578 | 591 |
|
|
| DDAO | 648 | 657 |
|
|
| DsRed | 559 | 583 |
|
|
| DsRed-Express | 556 | 584 |
|
|
| dTomato | 556 | 582 |
|
|
| DY-590 | 582 | 599 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| LysoTracker Red | 573 | 592 |
|
|
| mApple | 566 | 594 |
|
|
| mCherry | 587 | 610 |
|
|
| MitoTracker™ Red | 578 | 598 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP | 555 | 583 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mRuby | 558 | 605 |
|
|
| mStrawberry | 574 | 596 |
|
|
| mTangerine | 568 | 585 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| PerCP | 490 | 677 |
|
|
| pHrodo™, succinimidyl ester | 560 | 587 |
|
|
| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| Rhodamine Red-X | 573 | 591 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| SpectrumOrange | 554 | 587 |
|
|
| SpectrumRed | 587 | 615 |
|
|
| Sulforhodamine 101-EtOH | 578 | 593 |
|
|
| Tetramethylrhodamine dextran | 554 | 582 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red-X® | 595 | 613 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YOYO-3 | 611 | 631 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF02-641/75-25
FL-004436 / 0
- Tavg > 93% 604 – 679 nm
- Center Wavelength of 641.5 nm and GMBW of 75 nm
- FWHM of 81.7 nm
FF01-562/40-25
FL-004456 / 0
- Tavg > 93% 542 – 582 nm
- Center Wavelength of 562 nm and GMBW of 40 nm
- FWHM of 45.5 nm
FF593-Di03-25x36
FL-007026 / 0
- Dichroic Beamsplitter with edge at 593 nm
- Ravg > 98% 350 – 585 nm
- Tavg > 93% 601 – 950 nm
- Flatness / RWE: Standard Epi-fluorescence
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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