This filter set is ideal for use with the monomeric fluorescent protein mCherry. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide high brightness.
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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2-dodecylresorufin-lipid | 582 | 595 |
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5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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AsRed 2 | 576 | 592 |
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ATTO 565 | 563 | 592 |
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ATTO 590 | 594 | 624 |
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ATTO 594 | 601 | 624 |
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BOBO™-3 | 570 | 605 |
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BODIPY TR-X phallacidin | 590 | 621 |
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Calcium Crimson | 589 | 609 |
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Carboxynaphthofluorescein | 599 | 674 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTracker Red CMTPX | 586 | 614 |
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ChlorophyllA | 480 | 650 |
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Cy3.5™ | 578 | 591 |
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DDAO | 648 | 657 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-590 | 582 | 599 |
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DyLight 594 | 593 | 618 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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FM 4-64 | 505 | 725 |
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HcRed1 | 588 | 618 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluor™ 594 | 592 | 616 |
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KFP-Red | 575 | 599 |
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LDS 751 | 560 | 711 |
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LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
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LysoTracker Red | 573 | 592 |
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mApple | 566 | 594 |
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mCherry | 587 | 610 |
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MitoTracker™ Red | 578 | 598 |
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mKate2 | 588 | 633 |
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mPlum | 589 | 649 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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Nile red-phospolipid | 553 | 637 |
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PerCP | 490 | 677 |
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Propidium Iodide (PI) | 305 | 617 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Qdot® 625 Nanocrystals | 300 | 621 |
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Qdot® 655 Nanocrystals | 300 | 654 |
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Qdot® 705 Nanocrystals | 300 | 702 |
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Qdot® 800 Nanocrystals | 300 | 792 |
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ReAsH-CCPGCC | 592 | 606 |
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Red 580 | 580 | 603 |
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Rhodamine Red-X | 573 | 591 |
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SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 488nm (ph 9.0) | 576 | 638 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SNARF-1 514nm (ph 9.0) | 576 | 638 |
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SpectrumRed | 587 | 615 |
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Sulforhodamine 101-EtOH | 578 | 593 |
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Texas Red dextran | 592 | 613 |
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Texas Red DHPE | 584 | 608 |
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Texas Red® | 592 | 614 |
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TurboFP635(Katushka) | 591 | 638 |
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X-Rhod-1 Indicator | 580 | 601 |
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YOYO-3 | 611 | 631 |
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm The Nikon “true” cube needs a Di with corner chamfered (-QF) | 1.1 mm (Can accept a 2 mm dichroic) |