This filter set is optimized to be a high brightness and high signal-to-noise ratio (contrast).
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-538/40-25
FL-004341 / 0
- Tavg > 90% 518 – 558 nm
- Center Wavelength of 538 nm and GMBW of 40 nm
- FWHM of 45.2 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Orange (+DNA) | 502 | 526 |
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| Acridine Yellow | 470 | 550 |
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| Alexa Fluor® 430 | 431 | 541 |
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| ATTO 425 | 436 | 484 |
|
|
| BCECF (pH 5.5) | 481 | 518 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| Cerulean | 434 | 473 |
|
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| DiA | 457 | 586 |
|
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| FM 1-43 | 473 | 579 |
|
|
| Lucifer yellow | 428 | 544 |
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| LysoSensor Green | 448 | 502 |
|
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| mHoneyDew | 478 | 562 |
|
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| NBD-X (MeOH) | 467 | 538 |
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| Pacific Orange | 400 | 551 |
|
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| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| SYTO 45 | 451 | 485 |
|
|
| ThiolTracker Violet+GSH | 404 | 526 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-538/40-25
FL-004341 / 0
- Tavg > 90% 518 – 558 nm
- Center Wavelength of 538 nm and GMBW of 40 nm
- FWHM of 45.2 nm
FF02-438/24-25
FL-004649 / 0
- Tavg > 93% 426 – 450 nm
- Center Wavelength of 438 nm and GMBW of 24 nm
- FWHM of 29.5 nm
FF482-Di01-25x36
FL-006842 / 0
- Dichroic Beamsplitter with edge at 482 nm
- Ravg > 90% 415 – 470 nm
- Tavg > 90% 490 – 720 nm
- Flatness / RWE: Standard Epi-fluorescence
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LP03-532RE-25
FL-009094