This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 633 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF635/LP-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,754.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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| Center Wavelength 4 | 640 nm |
|---|---|
| Edge Wavelength 1 | 638 nm |
| Laser Wavelength 1 (low) | 632.8 nm, 647.1 nm |
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FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di02-R635-25x36
FL-007077 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Laser
BLP01-635R-25
FL-008533 / 0
- Filter with 50% edge at 655 nm
- 380 cm-1 transition
- Tavg > 93% from 660 – 1200 nm
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This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
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| Alexa Fluor® 633 | 631 | 647 |
|
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| Alexa Fluor® 635 | 633 | 648 |
|
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| Alexa Fluor® 647 | 653 | 668 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| Alexa Fluor® 680 | 679 | 702 |
|
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| Alexa Fluor® 700 | 696 | 719 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| ATTO 633 | 629 | 658 |
|
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| ATTO 635 | 635 | 658 |
|
|
| ATTO 647 | 644 | 670 |
|
|
| ATTO 647N | 645 | 668 |
|
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| ATTO 655 | 663 | 683 |
|
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| ATTO 680 | 680 | 700 |
|
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| ATTO 700 | 699 | 719 |
|
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| BODIPY 650/665-X | 646 | 664 |
|
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cy5.5™ | 672 | 690 |
|
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| Cy5™ | 649 | 666 |
|
|
| DDAO | 648 | 657 |
|
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
|
| DY-630 | 634 | 656 |
|
|
| DY-675 | 675 | 699 |
|
|
| DyLight 649 | 651 | 673 |
|
|
| DyLight 680 | 676 | 705 |
|
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
|
|
| HiLyte Fluor™ 680 | 688 | 702 |
|
|
| IRDye 680 NHS Ester | 683 | 710 |
|
|
| IRDye 700Phosphoramidite | 691 | 699 |
|
|
| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
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| MitoTracker™ Deep Red | 641 | 662 |
|
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| Nile blue-EtOH | 631 | 660 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
|
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| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| Quasar® 705 | 688 | 706 |
|
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| Red 650 | 649 | 665 |
|
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| SpectrumFRed (Far Red) | 650 | 673 |
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| SYTO 60 | 650 | 681 |
|
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| SYTO 61 | 618 | 650 |
|
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| SYTOX Red-DNA | 640 | 658 |
|
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| TO-PRO-3 | 642 | 657 |
|
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| TOTO-3 | 642 | 661 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di02-R635-25x36
FL-007077 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Laser
BLP01-635R-25
FL-008533 / 0
- Filter with 50% edge at 655 nm
- 380 cm-1 transition
- Tavg > 93% from 660 – 1200 nm
1 of 1
This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 21.8 mm | 5.0 mm |
| Emitter (Diameter) | 23.3 mm | 5.0 mm |
| Dichroic (L × W) | 22.0 mm × 29.0 mm | 1.1 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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