BrightLine® single-band 1λ P-V RWE super-resolution laser filter set, optimized for 635 nm laser sources

Semrock Part Number: LF635-C-ZHE

IDEX Part Number: S-003193  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 632.8 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF635-B

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,684.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Center Wavelength 4 640 nm
Edge Wavelength 1 638 nm
Laser Wavelength 1 (low) 632.8 nm, 647.1 nm

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661 – 690 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF01-640/14-25

FL-004697 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 633 – 647 nm
  • Center Wavelength of 640 nm and GMBW of 14 nm
  • FWHM of 20.2 nm

Di03-R635-t1-25x36

FL-006959 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 655.8 nm
  • Rabs > 94% 632.8 – 647.1 nm
  • Ravg > 90% 350 – 632.8 nm
  • Tavg > 93% 658.8 – 1200 nm
  • Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
APC (Allophycocyanin) 651 660
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
BODIPY 650/665-X 646 664
Carboxynaphthofluorescein 599 674
Cy5.5™ 672 690
Cy5™ 649 666
DDAO 648 657
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DY-630 634 656
DyLight 633 620 649
DyLight 649 651 673
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HiLyte Fluor™ 647 649 673
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
MitoTracker™ Deep Red 641 662
Nile blue-EtOH 631 660
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
SpectrumFRed (Far Red) 650 673
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTOX Red-DNA 640 658
TO-PRO-3 642 657
TOTO-3 642 661
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-676/29-25

FL-004567 / 0

Out of Stock Backordered In Stock
  • Tavg > 90% 661 – 690 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FF01-640/14-25

FL-004697 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 633 – 647 nm
  • Center Wavelength of 640 nm and GMBW of 14 nm
  • FWHM of 20.2 nm

Di03-R635-t1-25x36

FL-006959 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 655.8 nm
  • Rabs > 94% 632.8 – 647.1 nm
  • Ravg > 90% 350 – 632.8 nm
  • Tavg > 93% 658.8 – 1200 nm
  • Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.