BrightLine® single-band 1λ P-V RWE super-resolution laser filter set, optimized for 635 nm laser sources

Semrock Part Number: LF635-C-OFF

IDEX Part Number: S-003192  /

Semrock
1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: Cy5, Alexa Fluor® 647, APC, ATTO 647, BODIPY 650/665-X, MitoTracker™ Deep Red
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Filter set mounted in microscope holders/cubes found below in related products section.
Set Components

FF01-640/14-25

FL-004697

Each
  • Tavg > 93% 633 – 647 nm
  • Center Wavelength of 640 nm and GMBW of 14 nm
  • FWHM of 20.2 nm

FL-004697

FF01-676/29-25

FL-004567

Each
  • Tavg > 90% 661.5 – 690.5 nm
  • Center Wavelength of 676 nm and GMBW of 29 nm
  • FWHM of 36.1 nm

FL-004567

Di03-R635-t1

S-000995

Each

S-000995

Center Wavelength 4 640 nm
Edge Wavelength 1 638 nm
Laser Wavelength 1 (low) 632.8 nm, 647.1 nm

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63

Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
Alexa Fluor® 633 631 647
Alexa Fluor® 635 633 648
Alexa Fluor® 647 653 668
Alexa Fluor® 660 663 691
APC (Allophycocyanin) 651 660
ATTO 633 629 658
ATTO 635 635 658
ATTO 647 644 670
ATTO 647N 645 668
ATTO 655 663 683
BODIPY 650/665-X 646 664
Carboxynaphthofluorescein 599 674
Cy5.5™ 672 690
Cy5™ 649 666
DDAO 648 657
DiD (DiIC18(5)) - Lipophilic Tracer 648 670
DY-630 634 656
DyLight 633 620 649
DyLight 649 651 673
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Dark Red fluorescent microspheres 656 683
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HiLyte Fluor™ 647 649 673
LIVE-DEAD Fixable Far Red Dead Cell Stain 653 668
MitoTracker™ Deep Red 641 662
Nile blue-EtOH 631 660
Qdot® 705 Nanocrystals 300 702
Red 650 649 665
SpectrumFRed (Far Red) 650 673
SYTO 59 622 644
SYTO 60 650 681
SYTO 61 618 650
SYTOX Red-DNA 640 658
TO-PRO-3 642 657
TOTO-3 642 661

Specifications

This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63