This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 632.8 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF635-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,844.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
Filter set mounted in microscope holders/cubes found below in related products section.
Set Components
FF01-640/14-25FL-004697
Each
|
FL-004697 |
|
FF01-676/29-25FL-004567
Each
|
FL-004567 |
|
|
|
S-000995 |
| Center Wavelength 4 | 640 nm |
|---|---|
| Edge Wavelength 1 | 638 nm |
| Laser Wavelength 1 (low) | 632.8 nm, 647.1 nm |
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FF01-676/29-25
FL-004567 / 0
- Tavg > 90% 661 – 690 nm
- Center Wavelength of 676 nm and GMBW of 29 nm
- FWHM of 36.1 nm
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di03-R635-t1-25x36
FL-006959 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| Alexa Fluor® 633 | 631 | 647 |
|
|
| Alexa Fluor® 635 | 633 | 648 |
|
|
| Alexa Fluor® 647 | 653 | 668 |
|
|
| Alexa Fluor® 660 | 663 | 691 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| ATTO 633 | 629 | 658 |
|
|
| ATTO 635 | 635 | 658 |
|
|
| ATTO 647 | 644 | 670 |
|
|
| ATTO 647N | 645 | 668 |
|
|
| ATTO 655 | 663 | 683 |
|
|
| BODIPY 650/665-X | 646 | 664 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| Cy5.5™ | 672 | 690 |
|
|
| Cy5™ | 649 | 666 |
|
|
| DDAO | 648 | 657 |
|
|
| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
|
|
| DY-630 | 634 | 656 |
|
|
| DyLight 633 | 620 | 649 |
|
|
| DyLight 649 | 651 | 673 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
|
|
| HCS CellMask Red cytoplasmic | 622 | 644 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HiLyte Fluor™ 647 | 649 | 673 |
|
|
| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
|
|
| MitoTracker™ Deep Red | 641 | 662 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Red 650 | 649 | 665 |
|
|
| SpectrumFRed (Far Red) | 650 | 673 |
|
|
| SYTO 59 | 622 | 644 |
|
|
| SYTO 60 | 650 | 681 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| SYTOX Red-DNA | 640 | 658 |
|
|
| TO-PRO-3 | 642 | 657 |
|
|
| TOTO-3 | 642 | 661 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-676/29-25
FL-004567 / 0
- Tavg > 90% 661 – 690 nm
- Center Wavelength of 676 nm and GMBW of 29 nm
- FWHM of 36.1 nm
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di03-R635-t1-25x36
FL-006959 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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