This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 632.8 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF635-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,794.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
| Center Wavelength 4 | 640 nm |
|---|---|
| Edge Wavelength 1 | 638 nm |
| Laser Wavelength 1 (low) | 632.8 nm, 647.1 nm |
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FF01-676/29-25
FL-004567 / 0
- Tavg > 90% 661.5 – 690.5 nm
- Center Wavelength of 676 nm and GMBW of 29 nm
- FWHM of 36.1 nm
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di03-R635-t1-25x36
FL-006959 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| Alexa Fluor® 633 | 631 | 647 |
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| Alexa Fluor® 635 | 633 | 648 |
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| Alexa Fluor® 647 | 653 | 668 |
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| Alexa Fluor® 660 | 663 | 691 |
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| APC (Allophycocyanin) | 651 | 660 |
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| ATTO 633 | 629 | 658 |
|
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| ATTO 635 | 635 | 658 |
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| ATTO 647 | 644 | 670 |
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| ATTO 647N | 645 | 668 |
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| ATTO 655 | 663 | 683 |
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| BODIPY 650/665-X | 646 | 664 |
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| Cy5.5™ | 672 | 690 |
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| Cy5™ | 649 | 666 |
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| DDAO | 648 | 657 |
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| DiD (DiIC18(5)) - Lipophilic Tracer | 648 | 670 |
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| DY-630 | 634 | 656 |
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| DyLight 633 | 620 | 649 |
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| DyLight 649 | 651 | 673 |
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
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| FluoSpheres Dark Red fluorescent microspheres | 656 | 683 |
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HiLyte Fluor™ 647 | 649 | 673 |
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| LIVE-DEAD Fixable Far Red Dead Cell Stain | 653 | 668 |
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| MitoTracker™ Deep Red | 641 | 662 |
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| Nile blue-EtOH | 631 | 660 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Red 650 | 649 | 665 |
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| SpectrumFRed (Far Red) | 650 | 673 |
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| SYTO 59 | 622 | 644 |
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| SYTO 60 | 650 | 681 |
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| SYTO 61 | 618 | 650 |
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| SYTOX Red-DNA | 640 | 658 |
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| TO-PRO-3 | 642 | 657 |
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| TOTO-3 | 642 | 661 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-676/29-25
FL-004567 / 0
- Tavg > 90% 661.5 – 690.5 nm
- Center Wavelength of 676 nm and GMBW of 29 nm
- FWHM of 36.1 nm
FF01-640/14-25
FL-004697 / 0
- Tavg > 93% 633 – 647 nm
- Center Wavelength of 640 nm and GMBW of 14 nm
- FWHM of 20.2 nm
Di03-R635-t1-25x36
FL-006959 / 0
- Laser Dichroic Beamsplitter with edge at 655.8 nm
- Rabs > 94% 632.8 – 647.1 nm
- Ravg > 90% 350 – 632.8 nm
- Tavg > 93% 658.8 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
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