BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

Semrock Part Number: LF594/LP-D-OFF

IDEX Part Number: S-001848  /

Semrock

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF or LDMK?
Our previous version is still available for those cube configurations LF594/LP-C

  • Price Not Available.
  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 0.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • 0.00
    0.00
  • Notify me when available
Laser Wavelength 1 (high) 594.3 nm
Laser Wavelength 1 (low) 593.5 nm

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

Di03-R594-t1-25x36

FL-006944 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 599.5 nm
  • Rabs > 94% 593.5 – 594.3 nm
  • Ravg > 90% 350 – 593.5 nm
  • Tavg > 93% 605 – 1200 nm  
  • Flatness / RWE: Super-resolution / TIRF


BLP01-594R-25

FL-008542 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 607 nm
  • 411 cm-1 transition
  • Tavg > 93% from 610.9 – 900 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Alexa Fluor® 660 663 691
APC 651 660
APC (Allophycocyanin) 651 660
AsRed 2 576 592
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BOBO™-3 570 605
BODIPY 630/650-X 625 641
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
ChlorophyllA 480 650
DDAO 648 657
DY-590 582 599
DyLight 594 593 618
DyLight 633 620 649
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
FM 4-64 505 725
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
KFP-Red 575 599
LDS 751 560 711
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
mCherry 587 610
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP1 584 700
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
ReAsH-CCPGCC 592 606
Red 580 580 603
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
SpectrumRed 587 615
SYTO 17 619 638
SYTO 59 622 644
SYTO 61 618 650
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TurboFP635(Katushka) 591 638
X-Rhod-1 Indicator 580 601
YO-PRO-3 613 629
YOYO-3 611 631
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

Di03-R594-t1-25x36

FL-006944 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 599.5 nm
  • Rabs > 94% 593.5 – 594.3 nm
  • Ravg > 90% 350 – 593.5 nm
  • Tavg > 93% 605 – 1200 nm  
  • Flatness / RWE: Super-resolution / TIRF


BLP01-594R-25

FL-008542 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 607 nm
  • 411 cm-1 transition
  • Tavg > 93% from 610.9 – 900 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.