This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF594/LP-D
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LF594/LP-D-000
S-000906 / 0
1λ P-V RWE super-resolution laser optimized long-pass filter set. Ideal for use with: mCherry, mKate2, Alexa Fluor 594, Texas Red
LF594/LP-C-NQF
S-001844 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
LF594/LP-C-NTE
S-000488 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF594/LP-C-OMF
S-001846 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF594/LP-C-ZHE
S-001847 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF594/LP-C-OFF
S-001845 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| Alexa Fluor® 660 | 663 | 691 |
|
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| APC | 651 | 660 |
|
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| APC (Allophycocyanin) | 651 | 660 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
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| ATTO 594 | 601 | 624 |
|
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| ATTO 610 | 614 | 634 |
|
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| BOBOâ„¢-3 | 570 | 605 |
|
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| BODIPY 630/650-X | 625 | 641 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcium Crimson | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| ChlorophyllA | 480 | 650 |
|
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| DDAO | 648 | 657 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 594 | 593 | 618 |
|
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| DyLight 633 | 620 | 649 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
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| FM 4-64 | 505 | 725 |
|
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| HcRed1 | 588 | 618 |
|
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluorâ„¢ 594 | 592 | 616 |
|
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| KFP-Red | 575 | 599 |
|
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| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| mCherry | 587 | 610 |
|
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| MitoTrackerâ„¢ Red | 578 | 598 |
|
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| mKate2 | 588 | 633 |
|
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| mPlum | 589 | 649 |
|
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| mRFP1 | 584 | 700 |
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| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Qdot® 800 Nanocrystals | 300 | 792 |
|
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| SpectrumRed | 587 | 615 |
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| SYTO 17 | 619 | 638 |
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| SYTO 59 | 622 | 644 |
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| SYTO 61 | 618 | 650 |
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
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| Texas Red® | 592 | 614 |
|
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| TurboFP635(Katushka) | 591 | 638 |
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| X-Rhod-1 Indicator | 580 | 601 |
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| YO-PRO-3 | 613 | 629 |
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| YOYO-3 | 611 | 631 |
|
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