This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF or LDMK?
Our previous version is still available for those cube configurations LF594-C
-
EachUS Domestic pricing – contact your local distributor for other currency list prices
-
0.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
-
-
0.000.00 -
-
- Notify me when available
| Laser Wavelength 1 (high) | 594.3 nm |
|---|---|
| Laser Wavelength 1 (low) | 593.5 nm |
total selected
Clear selection
Select all 3 products from all pages.
FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
Di03-R594-t1-25x36
FL-006944 / 0
- Laser Dichroic Beamsplitter with edge at 599.5 nm
- Rabs > 94% 593.5 – 594.3 nm
- Ravg > 90% 350 – 593.5 nm
- Tavg > 93% 605 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
|
| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| APC | 651 | 660 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| ATTO 610 | 614 | 634 |
|
|
| BODIPY 630/650-X | 625 | 641 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
|
| CellTracker Red CMTPX | 586 | 614 |
|
|
| ChlorophyllA | 480 | 650 |
|
|
| DDAO | 648 | 657 |
|
|
| DyLight 594 | 593 | 618 |
|
|
| DyLight 633 | 620 | 649 |
|
|
| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| HcRed1 | 588 | 618 |
|
|
| HCS CellMask Red cytoplasmic | 622 | 644 |
|
|
| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
|
| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
|
| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
|
| HiLyte Fluor™ 594 | 592 | 616 |
|
|
| Hypericin | 600 | 603 |
|
|
| KFP-Red | 575 | 599 |
|
|
| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
|
| mApple | 566 | 594 |
|
|
| mCherry | 587 | 610 |
|
|
| mKate2 | 588 | 633 |
|
|
| mPlum | 589 | 649 |
|
|
| mRFP1 | 584 | 700 |
|
|
| mStrawberry | 574 | 596 |
|
|
| Nile blue-EtOH | 631 | 660 |
|
|
| Nile red-phospolipid | 553 | 637 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| ReAsH-CCPGCC | 592 | 606 |
|
|
| Red 580 | 580 | 603 |
|
|
| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
|
| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
|
| SpectrumRed | 587 | 615 |
|
|
| SYTO 17 | 619 | 638 |
|
|
| SYTO 59 | 622 | 644 |
|
|
| SYTO 61 | 618 | 650 |
|
|
| Texas Red dextran | 592 | 613 |
|
|
| Texas Red DHPE | 584 | 608 |
|
|
| Texas Red® | 592 | 614 |
|
|
| TurboFP635(Katushka) | 591 | 638 |
|
|
| X-Rhod-1 Indicator | 580 | 601 |
|
|
| YO-PRO-3 | 613 | 629 |
|
|
| YOYO-3 | 611 | 631 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
Di03-R594-t1-25x36
FL-006944 / 0
- Laser Dichroic Beamsplitter with edge at 599.5 nm
- Rabs > 94% 593.5 – 594.3 nm
- Ravg > 90% 350 – 593.5 nm
- Tavg > 93% 605 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
You might also be interested in the following products
Please contact your Partner with AVR Optics to purchase.
To purchase this product, click into product details for more information.
Adding...
Removing
Processing...
{{productContent.processedProductsCount}} of {{productContent.model.matrixProducts.length}}
Recently Viewed
View All
LPD02-785RU-25
FL-008049