BrightLine® single-band laser filter set, optimized for 594 nm laser sources

Semrock Part Number: LF594-C-OMF

IDEX Part Number: S-001133  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF594-D

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  • 1,774.00 USD Each
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Laser Wavelength 1 (high) 594.3 nm
Laser Wavelength 1 (low) 593.5 nm

FF01-647/57-25

FL-004142 / 0

Out of Stock Backordered In Stock
  • Tavg > 92% 618.5 – 675.5 nm
  • Center Wavelength of 647 nm and GMBW of 57 nm
  • FWHM of 70 nm

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

Di02-R594-25x36

FL-007017 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 599.5 nm
  • Rabs > 94% 593.5 – 594.3 nm
  • Ravg > 90% 350 – 593.5 nm
  • Tavg > 93% 605 – 1200 nm
  •  Flatness / RWE: Laser

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BODIPY 630/650-X 625 641
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
ChlorophyllA 480 650
DDAO 648 657
DyLight 594 593 618
DyLight 633 620 649
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
mApple 566 594
mCherry 587 610
mKate2 588 633
mPlum 589 649
mRFP1 584 700
mStrawberry 574 596
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
ReAsH-CCPGCC 592 606
Red 580 580 603
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
SpectrumRed 587 615
SYTO 17 619 638
SYTO 59 622 644
SYTO 61 618 650
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TurboFP635(Katushka) 591 638
X-Rhod-1 Indicator 580 601
YO-PRO-3 613 629
YOYO-3 611 631
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-647/57-25

FL-004142 / 0

Out of Stock Backordered In Stock
  • Tavg > 92% 618.5 – 675.5 nm
  • Center Wavelength of 647 nm and GMBW of 57 nm
  • FWHM of 70 nm

FF01-591/6-25

FL-004689 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 588 – 594.5 nm
  • Center Wavelength of 591 nm and GMBW of 6 nm
  • FWHM of 10.0 nm

Di02-R594-25x36

FL-007017 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 599.5 nm
  • Rabs > 94% 593.5 – 594.3 nm
  • Ravg > 90% 350 – 593.5 nm
  • Tavg > 93% 605 – 1200 nm
  •  Flatness / RWE: Laser

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.