This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF594-D
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,724.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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| Laser Wavelength 1 (high) | 594.3 nm |
|---|---|
| Laser Wavelength 1 (low) | 593.5 nm |
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FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
Di02-R594-25x36
FL-007017 / 0
- Laser Dichroic Beamsplitter with edge at 599.5 nm
- Rabs > 94% 593.5 – 594.3 nm
- Ravg > 90% 350 – 593.5 nm
- Tavg > 93% 605 – 1200 nm
- Flatness / RWE: Laser
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
|
|
| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
|
| Alexa Fluor® 594 | 590 | 618 |
|
|
| APC | 651 | 660 |
|
|
| APC (Allophycocyanin) | 651 | 660 |
|
|
| ATTO 590 | 594 | 624 |
|
|
| ATTO 594 | 601 | 624 |
|
|
| ATTO 610 | 614 | 634 |
|
|
| BODIPY 630/650-X | 625 | 641 |
|
|
| BODIPY TR-X phallacidin | 590 | 621 |
|
|
| Calcium Crimson | 589 | 609 |
|
|
| Carboxynaphthofluorescein | 599 | 674 |
|
|
| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
|
| ChlorophyllA | 480 | 650 |
|
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| DDAO | 648 | 657 |
|
|
| DyLight 594 | 593 | 618 |
|
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| DyLight 633 | 620 | 649 |
|
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| FluoSpheres Crimson fluorescent microspheres | 620 | 646 |
|
|
| FluoSpheres Red fluorescent microspheres | 577 | 607 |
|
|
| HcRed1 | 588 | 618 |
|
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| HCS CellMask Red cytoplasmic | 622 | 644 |
|
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| HCS LipidTOX Deep Red neutral lipid stain | 635 | 652 |
|
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
|
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
|
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| HiLyte Fluor™ 594 | 592 | 616 |
|
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| Hypericin | 600 | 603 |
|
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| KFP-Red | 575 | 599 |
|
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| LIVE-DEAD Fixable Red Dead Cell Stain | 595 | 613 |
|
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| mApple | 566 | 594 |
|
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| mCherry | 587 | 610 |
|
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| mKate2 | 588 | 633 |
|
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| mPlum | 589 | 649 |
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| mRFP1 | 584 | 700 |
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| mStrawberry | 574 | 596 |
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| Nile blue-EtOH | 631 | 660 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
|
| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| SpectrumRed | 587 | 615 |
|
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| SYTO 17 | 619 | 638 |
|
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| SYTO 59 | 622 | 644 |
|
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| SYTO 61 | 618 | 650 |
|
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| Texas Red® | 592 | 614 |
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| TurboFP635(Katushka) | 591 | 638 |
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-3 | 613 | 629 |
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| YOYO-3 | 611 | 631 |
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-647/57-25
FL-004142 / 0
- Tavg > 92% 618.5 – 675.5 nm
- Center Wavelength of 647 nm and GMBW of 57 nm
- FWHM of 70 nm
FF01-591/6-25
FL-004689 / 0
- Tavg > 93% 588 – 594.5 nm
- Center Wavelength of 591 nm and GMBW of 6 nm
- FWHM of 10.0 nm
Di02-R594-25x36
FL-007017 / 0
- Laser Dichroic Beamsplitter with edge at 599.5 nm
- Rabs > 94% 593.5 – 594.3 nm
- Ravg > 90% 350 – 593.5 nm
- Tavg > 93% 605 – 1200 nm
- Flatness / RWE: Laser
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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