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BrightLine® single-band laser filter set, optimized for 594 nm laser sources

Semrock Part Number: LF594-C-NQF

IDEX Part Number: S-001837  /

Semrock
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
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  • Price Not Available.
  • 1,724.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Laser Wavelength 1 (high) 594.1 nm
Laser Wavelength 1 (low) 593.5 nm

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxynapthofluorescein (pH 10) 555 615
5-ROX (carboxy-X-rhodamine) 578 604
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
APC 651 660
APC (Allophycocyanin) 651 660
ATTO 590 594 624
ATTO 594 601 624
ATTO 610 614 634
BODIPY 630/650-X 625 641
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
ChlorophyllA 480 650
DDAO 648 657
DyLight 594 593 618
DyLight 633 620 649
FluoSpheres Crimson fluorescent microspheres 620 646
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS CellMask Red cytoplasmic 622 644
HCS LipidTOX Deep Red neutral lipid stain 635 652
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Red Dead Cell Stain 595 613
mApple 566 594
mCherry 587 610
mKate2 588 633
mPlum 589 649
mRFP1 584 700
mStrawberry 574 596
Nile blue-EtOH 631 660
Nile red-phospolipid 553 637
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
ReAsH-CCPGCC 592 606
Red 580 580 603
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 9.0) 576 638
SpectrumRed 587 615
SYTO 17 619 638
SYTO 59 622 644
SYTO 61 618 650
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TurboFP635(Katushka) 591 638
X-Rhod-1 Indicator 580 601
YO-PRO-3 613 629
YOYO-3 611 631

Specifications

This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm
The Nikon “true” cube needs a Di with corner chamfered (-QF)		1.1 mm
(Can accept a 2mm dichroic)

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