BrightLine® long-pass laser filter set, optimized for 561 & 568 nm laser sources

Semrock Part Number: LF561/LP-C-OFF

IDEX Part Number: S-001834  /

Semrock

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF561/LP-D

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  • 1,774.00 USD Each
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Laser Wavelength 1 (high) 568.2 nm
Laser Wavelength 3 561.4 nm

FF01-561/14-25

FL-004455 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 554 – 568 nm
  • Center Wavelength of 561 nm and GMBW of 14 nm
  • FWHM of 20.8 nm

Di02-R561-25x36

FL-007137 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 573 nm
  • Rabs > 94% 554 – 568.2 nm
  • Ravg > 90% 350 – 554 nm
  • Tavg > 93% 582.4 – 1200 nm
  •  Flatness / RWE: Laser

BLP02-561R-25

FL-008551 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 572 nm
  • 434.5 cm-1 transition
  • Tavg > 93% from 577.1 – 900 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxynapthofluorescein (pH 10) 555 615
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 546 556 572
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BOBO™-3 570 605
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Carboxynaphthofluorescein 599 674
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
ChlorophyllA 480 650
Cy3.5™ 578 591
Cy3™ 554 566
DDAO 648 657
Dendra2 (Red) 554 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 594 593 618
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Red fluorescent microspheres 577 607
FM 4-64 505 725
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LDS 751 560 711
LOLO-1 568 580
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
Orange 552 552 574
PerCP 490 677
pHrodo™, succinimidyl ester 560 587
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYPRO Ruby protein gel stain 469 631
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboRFP 553 573
X-Rhod-1 Indicator 580 601
YOYO-3 611 631
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-561/14-25

FL-004455 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 554 – 568 nm
  • Center Wavelength of 561 nm and GMBW of 14 nm
  • FWHM of 20.8 nm

Di02-R561-25x36

FL-007137 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 573 nm
  • Rabs > 94% 554 – 568.2 nm
  • Ravg > 90% 350 – 554 nm
  • Tavg > 93% 582.4 – 1200 nm
  •  Flatness / RWE: Laser

BLP02-561R-25

FL-008551 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 572 nm
  • 434.5 cm-1 transition
  • Tavg > 93% from 577.1 – 900 nm