This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF561/LP-D
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,724.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 568.2 nm |
|---|---|
| Laser Wavelength 3 | 561.4 nm |
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FF01-561/14-25
FL-004455 / 0
- Tavg > 93% 554 – 568 nm
- Center Wavelength of 561 nm and GMBW of 14 nm
- FWHM of 20.8 nm
Di02-R561-25x36
FL-007137 / 0
- Laser Dichroic Beamsplitter with edge at 573 nm
- Rabs > 94% 554 – 568.2 nm
- Ravg > 90% 350 – 554 nm
- Tavg > 93% 582.4 – 1200 nm
- Flatness / RWE: Laser
BLP02-561R-25
FL-008551 / 0
- Filter with 50% edge at 572 nm
- 434.5 cm-1 transition
- Tavg > 93% from 577.1 – 900 nm
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-carboxynapthofluorescein (pH 10) | 555 | 615 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| AsRed 2 | 576 | 592 |
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcium Crimson | 589 | 609 |
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| Carboxynaphthofluorescein | 599 | 674 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| ChlorophyllA | 480 | 650 |
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| Cy3.5™ | 578 | 591 |
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| Cy3™ | 554 | 566 |
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| DDAO | 648 | 657 |
|
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| Dendra2 (Red) | 554 | 569 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-590 | 582 | 599 |
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| DyLight 594 | 593 | 618 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| FM 4-64 | 505 | 725 |
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| HiLyte Fluor™ 594 | 592 | 616 |
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| Hypericin | 600 | 603 |
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| KFP-Red | 575 | 599 |
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| LDS 751 | 560 | 711 |
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| LOLO-1 | 568 | 580 |
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| LysoTracker Red | 573 | 592 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
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| mKate2 | 588 | 633 |
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| mPlum | 589 | 649 |
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| mRFP | 555 | 583 |
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile red-phospolipid | 553 | 637 |
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| Orange 552 | 552 | 574 |
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| PerCP | 490 | 677 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| Propidium Iodide (PI) | 305 | 617 |
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| Qdot® 655 Nanocrystals | 300 | 654 |
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| Qdot® 705 Nanocrystals | 300 | 702 |
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| Qdot® 800 Nanocrystals | 300 | 792 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine phalloidin | 558 | 575 |
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| Rhodamine Red-X | 573 | 591 |
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| SpectrumOrange | 554 | 587 |
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| Texas Red® | 592 | 614 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboFP635(Katushka) | 591 | 638 |
|
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| TurboRFP | 553 | 573 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YOYO-3 | 611 | 631 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-561/14-25
FL-004455 / 0
- Tavg > 93% 554 – 568 nm
- Center Wavelength of 561 nm and GMBW of 14 nm
- FWHM of 20.8 nm
Di02-R561-25x36
FL-007137 / 0
- Laser Dichroic Beamsplitter with edge at 573 nm
- Rabs > 94% 554 – 568.2 nm
- Ravg > 90% 350 – 554 nm
- Tavg > 93% 582.4 – 1200 nm
- Flatness / RWE: Laser
BLP02-561R-25
FL-008551 / 0
- Filter with 50% edge at 572 nm
- 434.5 cm-1 transition
- Tavg > 93% from 577.1 – 900 nm