This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF561-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,682.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 568.2 nm |
|---|---|
| Laser Wavelength 3 | 561.4 nm |
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FF01-561/14-25
FL-004455 / 0
- Tavg > 93% 554 – 568 nm
- Center Wavelength of 561 nm and GMBW of 14 nm
- FWHM of 20.8 nm
FF01-609/54-25
FL-004693 / 0
- Tavg > 93% 582 – 636 nm
- Center Wavelength of 609 nm and GMBW of 54 nm
- FWHM of 60.6 nm
Di03-R561-t1-25x36
FL-007140 / 0
- Laser Dichroic Beamsplitter with edge at 576.2 nm
- Rabs > 94% 554 – 568.2 nm
- Ravg > 90% 350 – 554 nm
- Tavg > 93% 582.4 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
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| Alexa Fluor® 546 | 556 | 572 |
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| Alexa Fluor® 555 | 553 | 565 |
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| Alexa Fluor® 568 | 579 | 603 |
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| Alexa Fluor® 594 | 590 | 618 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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| AsRed 2 | 576 | 592 |
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| ATTO 550 | 553 | 576 |
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| ATTO 565 | 563 | 592 |
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| ATTO 590 | 594 | 624 |
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| BOBO™-3 | 570 | 605 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Orange | 589 | 609 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTracker Red CMTPX | 586 | 614 |
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| Cy3.5™ | 578 | 591 |
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| Cy3™ | 554 | 566 |
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| Dendra2 (Red) | 554 | 569 |
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| DiI | 551 | 569 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-590 | 582 | 599 |
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| DyLight 549 | 553 | 569 |
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| DyLight 594 | 593 | 618 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| HiLyte Fluor™ 594 | 592 | 616 |
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| Hypericin | 600 | 603 |
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| KFP-Red | 575 | 599 |
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| LOLO-1 | 568 | 580 |
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| LysoTracker Red | 573 | 592 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
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| mKate2 | 588 | 633 |
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| mOrange2 | 551 | 565 |
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| mPlum | 589 | 649 |
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| mRFP | 555 | 583 |
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| Nile red-phospolipid | 553 | 637 |
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| OFP | 547 | 567 |
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| Orange 552 | 552 | 574 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| Propidium Iodide (PI) | 305 | 617 |
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 585 Nanocrystals | 300 | 588 |
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine phalloidin | 558 | 575 |
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| Rhodamine Red-X | 573 | 591 |
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| R-phycoerythrin (PE) | 565 | 576 |
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| SpectrumOrange | 554 | 587 |
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| Sulforhodamine 101-EtOH | 578 | 593 |
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| SYPRO Ruby protein gel stain | 469 | 631 |
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| Tetramethylrhodamine dextran | 554 | 582 |
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| Texas Red dextran | 592 | 613 |
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| Texas Red DHPE | 584 | 608 |
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| Texas Red® | 592 | 614 |
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
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| TurboFP635(Katushka) | 591 | 638 |
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| TurboRFP | 553 | 573 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
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| YOYO-3 | 611 | 631 |
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-561/14-25
FL-004455 / 0
- Tavg > 93% 554 – 568 nm
- Center Wavelength of 561 nm and GMBW of 14 nm
- FWHM of 20.8 nm
FF01-609/54-25
FL-004693 / 0
- Tavg > 93% 582 – 636 nm
- Center Wavelength of 609 nm and GMBW of 54 nm
- FWHM of 60.6 nm
Di03-R561-t1-25x36
FL-007140 / 0
- Laser Dichroic Beamsplitter with edge at 576.2 nm
- Rabs > 94% 554 – 568.2 nm
- Ravg > 90% 350 – 554 nm
- Tavg > 93% 582.4 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF