This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube [NTE, OFF, OMF or ZHE] mounted filter sets LF561-C
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Laser Wavelength 1 (high) | 568.2 nm |
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Laser Wavelength 3 | 561.4 nm |
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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2-dodecylresorufin-lipid | 582 | 595 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 555 | 553 | 565 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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AsRed 2 | 576 | 592 |
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ATTO 550 | 553 | 576 |
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ATTO 565 | 563 | 592 |
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ATTO 590 | 594 | 624 |
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BOBOâ„¢-3 | 570 | 605 |
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BODIPY TMR-X | 544 | 573 |
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BODIPY TR-X (MeOH) | 544 | 573 |
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BODIPY TR-X phallacidin | 590 | 621 |
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Calcium Crimson | 589 | 609 |
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Calcium Orange | 589 | 609 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTracker Red CMTPX | 586 | 614 |
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Cy3.5â„¢ | 578 | 591 |
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Cy3â„¢ | 554 | 566 |
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Dendra2 (Red) | 554 | 569 |
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DiI | 551 | 569 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-590 | 582 | 599 |
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DyLight 549 | 553 | 569 |
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DyLight 594 | 593 | 618 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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HcRed1 | 588 | 618 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluorâ„¢ 594 | 592 | 616 |
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Hypericin | 600 | 603 |
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KFP-Red | 575 | 599 |
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LOLO-1 | 568 | 580 |
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LysoTracker Red | 573 | 592 |
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Magnesium Orange | 550 | 574 |
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mApple | 566 | 594 |
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mCherry | 587 | 610 |
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Merocyanine 540 | 559 | 579 |
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MitoTrackerâ„¢ Orange | 551 | 575 |
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MitoTrackerâ„¢ Red | 578 | 598 |
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mKate2 | 588 | 633 |
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mOrange2 | 551 | 565 |
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mPlum | 589 | 649 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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Nile red-phospolipid | 553 | 637 |
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OFP | 547 | 567 |
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Orange 552 | 552 | 574 |
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pHrodoâ„¢, succinimidyl ester | 560 | 587 |
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Propidium Iodide (PI) | 305 | 617 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 585 Nanocrystals | 300 | 588 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Qdot® 625 Nanocrystals | 300 | 621 |
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ReAsH-CCPGCC | 592 | 606 |
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Red 580 | 580 | 603 |
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Resorufin | 571 | 584 |
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Rhod-2 | 553 | 577 |
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Rhodamine phalloidin | 558 | 575 |
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Rhodamine Red-X | 573 | 591 |
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R-phycoerythrin (PE) | 565 | 576 |
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SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 488nm (ph 9.0) | 576 | 638 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SNARF-1 514nm (ph 9.0) | 576 | 638 |
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SpectrumOrange | 554 | 587 |
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Sulforhodamine 101-EtOH | 578 | 593 |
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SYPRO Ruby protein gel stain | 469 | 631 |
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Tetramethylrhodamine dextran | 554 | 582 |
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Texas Red dextran | 592 | 613 |
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Texas Red DHPE | 584 | 608 |
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Texas Red® | 592 | 614 |
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TRITC (Tetramethylrhodamine) | 552 | 578 |
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TurboFP635(Katushka) | 591 | 638 |
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TurboRFP | 553 | 573 |
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X-Rhod-1 Indicator | 580 | 601 |
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YOYO-3 | 611 | 631 |
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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 3.5 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |