BrightLine® single-band laser filter set, optimized for 561 & 568 nm laser sources

Semrock Part Number: LF561-B-NTE

IDEX Part Number: S-000485  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube [NTE, OFF, OMF or ZHE] mounted filter sets LF561-C

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Laser Wavelength 1 (high) 568.2 nm
Laser Wavelength 3 561.4 nm

FF01-561/14-25

FL-004455 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 554 – 568 nm
  • Center Wavelength of 561 nm and GMBW of 14 nm
  • FWHM of 20.8 nm

FF01-609/54-25

FL-004693 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 582 – 636 nm
  • Center Wavelength of 609 nm and GMBW of 54 nm
  • FWHM of 60.6 nm

Di02-R561-25x36

FL-007137 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 573 nm
  • Rabs > 94% 554 – 568.2 nm
  • Ravg > 90% 350 – 554 nm
  • Tavg > 93% 582.4 – 1200 nm
  •  Flatness / RWE: Laser

1 of 1

This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
7-AAD (7-aminoactinomycin D) 548 648
Alexa Fluor® 546 556 572
Alexa Fluor® 555 553 565
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BOBOâ„¢-3 570 605
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcium Crimson 589 609
Calcium Orange 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
Cy3.5â„¢ 578 591
Cy3â„¢ 554 566
Dendra2 (Red) 554 569
DiI 551 569
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-590 582 599
DyLight 549 553 569
DyLight 594 593 618
Ethidium bromide 518 603
Ethidium homodimer 527 617
FluoSpheres Red fluorescent microspheres 577 607
HcRed1 588 618
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluorâ„¢ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LOLO-1 568 580
LysoTracker Red 573 592
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
MitoTrackerâ„¢ Orange 551 575
MitoTrackerâ„¢ Red 578 598
mKate2 588 633
mOrange2 551 565
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
Nile red-phospolipid 553 637
OFP 547 567
Orange 552 552 574
pHrodoâ„¢, succinimidyl ester 560 587
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577
Rhodamine phalloidin 558 575
Rhodamine Red-X 573 591
R-phycoerythrin (PE) 565 576
SNARF (carboxy) 514 Excitation pH9 576 638
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 488nm (ph 9.0) 576 638
SNARF-1 514nm (ph 6.0) 549 587
SNARF-1 514nm (ph 9.0) 576 638
SpectrumOrange 554 587
Sulforhodamine 101-EtOH 578 593
SYPRO Ruby protein gel stain 469 631
Tetramethylrhodamine dextran 554 582
Texas Red dextran 592 613
Texas Red DHPE 584 608
Texas Red® 592 614
TRITC (Tetramethylrhodamine) 552 578
TurboFP635(Katushka) 591 638
TurboRFP 553 573
X-Rhod-1 Indicator 580 601
YOYO-3 611 631
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-561/14-25

FL-004455 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 554 – 568 nm
  • Center Wavelength of 561 nm and GMBW of 14 nm
  • FWHM of 20.8 nm

FF01-609/54-25

FL-004693 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 582 – 636 nm
  • Center Wavelength of 609 nm and GMBW of 54 nm
  • FWHM of 60.6 nm

Di02-R561-25x36

FL-007137 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 573 nm
  • Rabs > 94% 554 – 568.2 nm
  • Ravg > 90% 350 – 554 nm
  • Tavg > 93% 582.4 – 1200 nm
  •  Flatness / RWE: Laser