BrightLine® single-band 1λ P-V RWE super-resolution laser filter set, optimized for 514 nm laser sources

Semrock Part Number: LF514-C-ZHE

IDEX Part Number: S-000483  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from YFP and other similar fluorophores while offering superb blocking of popular 515 nm (solid-state) or 514.5 nm (gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF514-B

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  • Each
    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 1,682.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Center Wavelength 1 514.5 nm
Center Wavelength 2 515 nm

FF02-510/10-25

FL-003881 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 505 – 515 nm
  • Center Wavelength of 510 nm and GMBW of 10 nm
  • FWHM of 15 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

Di03-R514-t1-25x36

FL-006920 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 520 nm
  • Rabs > 94% 505 – 515 nm
  • Ravg > 90% 350 – 505 nm
  • Tavg > 93% 524.3 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
6-carboxyrhodamine 6G 525 555
6-JOE 520 548
Acridine Orange (+DNA) 502 526
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 514 518 543
Alexa Fluor® 532 534 553
ATTO 488 500 525
ATTO 495 495 527
ATTO 520 516 538
ATTO 532 501 545
BCECF (pH 9.0) 502 528
BODIPY R6G 528 547
Calcium Green-1 507 529
CyQUANT GR-DNA 502 523
Dronpa 502 516
DY-505-Phalloidin 503 530
DyLight 488 493 518
Eosin 525 546
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
Gold 525 521 551
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JO-JO-1, JO-PRO-1 530 544
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
Magnesium Green 507 531
mHoneyDew 478 562
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboYFP 525 538
Venus 516 528
VIC 530 550
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
YFP (yellow GFP) 513 530
ZsYellow1 529 539
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF02-510/10-25

FL-003881 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 505 – 515 nm
  • Center Wavelength of 510 nm and GMBW of 10 nm
  • FWHM of 15 nm

FF01-542/27-25

FL-004609 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 528.5 – 555.5 nm
  • Center Wavelength of 542 nm and GMBW of 27 nm
  • FWHM of 33.1 nm

Di03-R514-t1-25x36

FL-006920 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 520 nm
  • Rabs > 94% 505 – 515 nm
  • Ravg > 90% 350 – 505 nm
  • Tavg > 93% 524.3 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

1 of 1

This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.