This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from YFP and other similar fluorophores while offering superb blocking of popular 515 nm (solid-state) or 514.5 nm (gas) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF514-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,869.63 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Center Wavelength 1 | 514.5 nm |
|---|---|
| Center Wavelength 2 | 515 nm |
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FF02-510/10-25
FL-003881 / 0
- Tavg > 93% 505 – 515 nm
- Center Wavelength of 510 nm and GMBW of 10 nm
- FWHM of 15 nm
FF01-542/27-25
FL-004609 / 0
- Tavg > 93% 528.5 – 555.5 nm
- Center Wavelength of 542 nm and GMBW of 27 nm
- FWHM of 33.1 nm
Di03-R514-t1-25x36
FL-006920 / 0
- Laser Dichroic Beamsplitter with edge at 520 nm
- Rabs > 94% 505 – 515 nm
- Ravg > 90% 350 – 505 nm
- Tavg > 93% 524.3 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 6-carboxyrhodamine 6G | 525 | 555 |
|
|
| 6-JOE | 520 | 548 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 514 | 518 | 543 |
|
|
| Alexa Fluor® 532 | 534 | 553 |
|
|
| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
|
| ATTO 520 | 516 | 538 |
|
|
| ATTO 532 | 501 | 545 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BODIPY R6G | 528 | 547 |
|
|
| Calcium Green-1 | 507 | 529 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| Dronpa | 502 | 516 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
|
| DyLight 488 | 493 | 518 |
|
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| Eosin | 525 | 546 |
|
|
| FITC (Fluorescein) | 495 | 519 |
|
|
| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
|
| Fluorescein dextran | 501 | 524 |
|
|
| Fluorescein-pH 8.0 | 489 | 517 |
|
|
| Fluoro-Emerald | 494 | 518 |
|
|
| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| Gold 525 | 521 | 551 |
|
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| Green 496 | 496 | 520 |
|
|
| Green 500 | 501 | 524 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| JO-JO-1, JO-PRO-1 | 530 | 544 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
|
| Magnesium Green | 507 | 531 |
|
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| mHoneyDew | 478 | 562 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
|
| Oregon Green™ 488 | 498 | 526 |
|
|
| Oregon Green™ 514 | 513 | 532 |
|
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| PicoGreen® | 502 | 522 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| Sodium Green | 507 | 532 |
|
|
| SpectrumGreen | 497 | 538 |
|
|
| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
|
| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TO-PRO-1 | 515 | 531 |
|
|
| TOTO-1 | 514 | 531 |
|
|
| TurboYFP | 525 | 538 |
|
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| Venus | 516 | 528 |
|
|
| VIC | 530 | 550 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| YFP (yellow GFP) | 513 | 530 |
|
|
| ZsYellow1 | 529 | 539 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
total selected
Clear selection
Select all 3 products from all pages.
FF02-510/10-25
FL-003881 / 0
- Tavg > 93% 505 – 515 nm
- Center Wavelength of 510 nm and GMBW of 10 nm
- FWHM of 15 nm
FF01-542/27-25
FL-004609 / 0
- Tavg > 93% 528.5 – 555.5 nm
- Center Wavelength of 542 nm and GMBW of 27 nm
- FWHM of 33.1 nm
Di03-R514-t1-25x36
FL-006920 / 0
- Laser Dichroic Beamsplitter with edge at 520 nm
- Rabs > 94% 505 – 515 nm
- Ravg > 90% 350 – 505 nm
- Tavg > 93% 524.3 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
1 of 1
This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
| Link | Description |
| Request a Drawing | Looking for an optical filter drawing file for this product? Request one now! |
| Request Custom Optical Filters | Contact us today to start a conversation about custom optical filters. |
| Download Optical Filters 101 Guide | Find an in-depth overview of optical filter principles, specifications, and applications. Request a digital copy of this guide now. |
| Request Clean Filter Checklist Poster | Looking to clean your optical filters? Need instructions? Request a printed copy of our Clean Filter Checklist Poster. |
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