This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from YFP and other similar fluorophores while offering superb blocking of popular 515 nm (solid-state) or 514.5 nm (gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF514-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,694.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Center Wavelength 1 | 514.5 nm |
|---|---|
| Center Wavelength 2 | 515 nm |
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FF02-510/10-25
FL-003881 / 0
- Tavg > 93% 505 – 515 nm
- Center Wavelength of 510 nm and GMBW of 10 nm
- FWHM of 15 nm
FF01-542/27-25
FL-004609 / 0
- Tavg > 93% 528.5 – 555.5 nm
- Center Wavelength of 542 nm and GMBW of 27 nm
- FWHM of 33.1 nm
Di02-R514-25x36
FL-007076 / 0
- Laser Dichroic Beamsplitter with edge at 520 nm
- Rabs > 94% 505 – 515 nm
- Ravg > 90% 350 – 505 nm
- Tavg > 93% 524.3 – 1200 nm
- Flatness / RWE: Laser
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This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| 6-JOE | 520 | 548 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| Alexa Fluor® 514 | 518 | 543 |
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| Alexa Fluor® 532 | 534 | 553 |
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 520 | 516 | 538 |
|
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| ATTO 532 | 501 | 545 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BODIPY R6G | 528 | 547 |
|
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| Calcium Green-1 | 507 | 529 |
|
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| CyQUANT GR-DNA | 502 | 523 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DyLight 488 | 493 | 518 |
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| Eosin | 525 | 546 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
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| Fluo-3 | 506 | 527 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
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| Gold 525 | 521 | 551 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
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| HiLyte Fluor™ 488 | 497 | 526 |
|
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| JO-JO-1, JO-PRO-1 | 530 | 544 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| Magnesium Green | 507 | 531 |
|
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| mHoneyDew | 478 | 562 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboYFP | 525 | 538 |
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| Venus | 516 | 528 |
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| VIC | 530 | 550 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| Vybrant DyeCycle Orange | 519 | 563 |
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| YFP (yellow GFP) | 513 | 530 |
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| ZsYellow1 | 529 | 539 |
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF02-510/10-25
FL-003881 / 0
- Tavg > 93% 505 – 515 nm
- Center Wavelength of 510 nm and GMBW of 10 nm
- FWHM of 15 nm
FF01-542/27-25
FL-004609 / 0
- Tavg > 93% 528.5 – 555.5 nm
- Center Wavelength of 542 nm and GMBW of 27 nm
- FWHM of 33.1 nm
Di02-R514-25x36
FL-007076 / 0
- Laser Dichroic Beamsplitter with edge at 520 nm
- Rabs > 94% 505 – 515 nm
- Ravg > 90% 350 – 505 nm
- Tavg > 93% 524.3 – 1200 nm
- Flatness / RWE: Laser
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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