This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF488/LP-C
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,365.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
BLP01-488R-25FL-008552
Each
|
FL-008552 |
|
Di03-R488-t1-25x36FL-007080
Each
|
FL-007080 |
|
FF02-482/18-25FL-004712
Each
|
FL-004712 |
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LF488/LP-D-NTE
S-000472 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF488/LP-D-OMF
S-000473 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
LF488/LP-D-ZHE
S-003149 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
LF488/LP-D-OFF
S-000897 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Olympus U-FF for microscopes: BX53, BX63
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
|
| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 6-carboxyrhodamine 6G | 525 | 555 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
|
| Acridine Orange (+DNA) | 502 | 526 |
|
|
| Acridine Orange (+RNA) | 460 | 650 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
|
| Alexa Fluor® 500 | 503 | 525 |
|
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| AmCyan1 | 458 | 489 |
|
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| ATTO 488 | 500 | 525 |
|
|
| ATTO 495 | 495 | 527 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
|
| BCECF (pH 9.0) | 502 | 528 |
|
|
| BODIPY FL | 505 | 512 |
|
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| BODIPY FL-MeOH | 502 | 511 |
|
|
| Calcein | 494 | 514 |
|
|
| Calcium Green-1 | 507 | 529 |
|
|
| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| CyQUANT GR-DNA | 502 | 523 |
|
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| DAF-FM-NO | 495 | 519 |
|
|
| Dendra2 (Green) | 491 | 507 |
|
|
| Dendra2 (Red) | 554 | 569 |
|
|
| Di-8-ANEPPS | 467 | 631 |
|
|
| DiA | 457 | 586 |
|
|
| DiO | 475 | 500 |
|
|
| Dronpa | 502 | 516 |
|
|
| DsRed-Express | 556 | 584 |
|
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| DY-505-Phalloidin | 503 | 530 |
|
|
| ecliptic pHluorin pH5.5 | 473 | 507 |
|
|
| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
|
| Ethidium homodimer | 527 | 617 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
|
|
| Fluo-3 | 506 | 527 |
|
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| Fluo-4 | 494 | 516 |
|
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| Fluorescein dextran | 501 | 524 |
|
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| Fluorescein-pH 8.0 | 489 | 517 |
|
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| Fluoro-Emerald | 494 | 518 |
|
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
|
|
| FM 1-43 | 473 | 579 |
|
|
| FM 4-64 | 505 | 725 |
|
|
| GFP (EGFP) | 489 | 511 |
|
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| Green 496 | 496 | 520 |
|
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| Green 500 | 501 | 524 |
|
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
|
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
|
|
| HiLyte Fluor™ 488 | 497 | 526 |
|
|
| JC-1 | 499 | 525 |
|
|
| LDS 751 | 560 | 711 |
|
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LysoSensor Green | 448 | 502 |
|
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Yellow HCK-123 | 488 | 565 |
|
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| Magnesium Green | 507 | 531 |
|
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| mHoneyDew | 478 | 562 |
|
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| MitoTracker™ Green | 490 | 512 |
|
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| mWasabi | 493 | 509 |
|
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-triglyceride | 510 | 583 |
|
|
| OFP | 547 | 567 |
|
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| Oregon Green™ 488 | 498 | 526 |
|
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| Oregon Green™ 514 | 513 | 532 |
|
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| PerCP | 490 | 677 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| ratiometric pHluorin pH5 | 500 | 509 |
|
|
| Rhodamine 110 | 497 | 519 |
|
|
| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodol Green | 497 | 524 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
|
| rsGFP (red shifted GFP, S65T) | 498 | 516 |
|
|
| sgGFP™ (super glow GFP) | 472 | 506 |
|
|
| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
|
| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
|
| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
|
| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
|
| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
|
| SYTO 9 | 483 | 500 |
|
|
| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
|
| SYTOX Green-DNA | 504 | 524 |
|
|
| TO-PRO-1 | 515 | 531 |
|
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| TOTO-1 | 514 | 531 |
|
|
| TurboGFP | 482 | 503 |
|
|
| Vybrant DyeCycle Green | 506 | 534 |
|
|
| Vybrant DyeCycle Orange | 519 | 563 |
|
|
| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
|
| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
total selected
Clear selection
Select all 4 products from all pages.
LF488/LP-D-NTE
S-000472 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator