BrightLine® long-pass laser filter set, optimized for 488 nm laser sources

Semrock Part Number: LF488/LP-C-LDMK

IDEX Part Number: S-001817  /

Semrock

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488/LP-D

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  • 1,704.00 USD Each
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Laser Wavelength 1 (high) 491 nm
Laser Wavelength 1 (low) 473 nm
Laser Wavelength 2 488 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di02-R488-25x36

FL-007135 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 495.5 nm
  • Rabs > 94% 471 – 491 nm
  • Ravg > 90% 350 – 471 nm
  • Tavg > 93% 499.8 – 900 nm
  • Flatness / RWE: Laser

Custom sizing delivery confirmed ARO (sizing fee applies).

BLP01-488R-25

FL-008552 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 500nm
  • 497 cm-1 transition
  • Tavg > 93% from 504.7 – 900nm

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Orange (+RNA) 460 650
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
Di-8-ANEPPS 467 631
DiA 457 586
DiO 475 500
Dronpa 502 516
DsRed-Express 556 584
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JC-1 499 525
LDS 751 560 711
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-triglyceride 510 583
OFP 547 567
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PerCP 490 677
PicoGreen® 502 522
PKH67 488 500
Propidium Iodide (PI) 305 617
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
R-phycoerythrin (PE) 565 576
rsGFP (red shifted GFP, S65T) 498 516
sgGFP™ (super glow GFP) 472 506
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYPRO Ruby protein gel stain 469 631
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di02-R488-25x36

FL-007135 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 495.5 nm
  • Rabs > 94% 471 – 491 nm
  • Ravg > 90% 350 – 471 nm
  • Tavg > 93% 499.8 – 900 nm
  • Flatness / RWE: Laser

Custom sizing delivery confirmed ARO (sizing fee applies).

BLP01-488R-25

FL-008552 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 500nm
  • 497 cm-1 transition
  • Tavg > 93% from 504.7 – 900nm