BrightLine® long-pass laser filter set, optimized for 488 nm laser sources

Semrock Part Number: LF488/LP-C-LDMK

IDEX Part Number: S-001817  /

Semrock

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488/LP-D

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  • 1,704.00 USD Each
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Laser Wavelength 1 (high) 491 nm
Laser Wavelength 1 (low) 473 nm
Laser Wavelength 2 488 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di02-R488-25x36

FL-007135 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 495.5 nm
  • Rabs > 94% 471 – 491 nm
  • Ravg > 90% 350 – 471 nm
  • Tavg > 93% 499.8 – 900 nm
  • Flatness / RWE: Laser

Custom sizing delivery confirmed ARO (sizing fee applies).

BLP01-488R-25

FL-008552 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 500nm
  • 497 cm-1 transition
  • Tavg > 93% from 504.7 – 900nm

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Acridine Orange (+RNA) 460 650
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
Di-8-ANEPPS 467 631
DiA 457 586
DiO 475 500
Dronpa 502 516
DsRed-Express 556 584
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
FM 4-64 505 725
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
JC-1 499 525
LDS 751 560 711
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-triglyceride 510 583
OFP 547 567
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PerCP 490 677
PicoGreen® 502 522
PKH67 488 500
Propidium Iodide (PI) 305 617
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
R-phycoerythrin (PE) 565 576
rsGFP (red shifted GFP, S65T) 498 516
sgGFP™ (super glow GFP) 472 506
SNARF-1 488nm (ph 6.0) 548 586
SNARF-1 514nm (ph 6.0) 549 587
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYPRO Ruby protein gel stain 469 631
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
Vybrant DyeCycle Orange 519 563
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508

Specifications

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di02-R488-25x36

FL-007135 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 495.5 nm
  • Rabs > 94% 471 – 491 nm
  • Ravg > 90% 350 – 471 nm
  • Tavg > 93% 499.8 – 900 nm
  • Flatness / RWE: Laser

Custom sizing delivery confirmed ARO (sizing fee applies).

BLP01-488R-25

FL-008552 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 500nm
  • 497 cm-1 transition
  • Tavg > 93% from 504.7 – 900nm