This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= λ/2 PV per inch @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488-D
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,694.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 491 nm |
|---|---|
| Laser Wavelength 1 (low) | 473 nm |
| Laser Wavelength 2 | 488 nm |
total selected
Clear selection
Select all 3 products from all pages.
FF01-525/45-25
FL-004680 / 0
- Tavg > 93% 502.5 – 547.5 nm
- Center Wavelength of 525 nm and GMBW of 45 nm
- FWHM of 50.6 nm
FF02-482/18-25
FL-004712 / 0
- Tavg > 93% 473 – 491 nm
- Center Wavelength of 482 nm and GMBW of 18 nm
- FWHM of 24.4 nm
Di02-R488-25x36
FL-007135 / 0
- Laser Dichroic Beamsplitter with edge at 495.5 nm
- Rabs > 94% 471 – 491 nm
- Ravg > 90% 350 – 471 nm
- Tavg > 93% 499.8 – 900 nm
- Flatness / RWE: Laser
Custom sizing delivery confirmed ARO (sizing fee applies).
1 of 1
This set is mounted in a Nikon NQF Quadfluor Fluorescence Filter Holder, which is compatible with Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE 200, TE 300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept a 2 mm dichroic) |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 6-carboxyrhodamine 6G | 525 | 555 |
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| Acridine Orange (+DNA) | 502 | 526 |
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
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| AmCyan1 | 458 | 489 |
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| ATTO 488 | 500 | 525 |
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| ATTO 495 | 495 | 527 |
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| BCECF (pH 5.5) | 481 | 518 |
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| BCECF (pH 9.0) | 502 | 528 |
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| Calcein | 494 | 514 |
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| Calcium Green-1 | 507 | 529 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
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| DiA | 457 | 586 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DY-505-Phalloidin | 503 | 530 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| evoglow-Bs1 | 449 | 496 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| FM 1-43 | 473 | 579 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| PicoGreen® | 502 | 522 |
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| PKH67 | 488 | 500 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
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| ratiometric pHluorin pH5 | 500 | 509 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
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| Rhodamine Green | 497 | 523 |
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| Rhodol Green | 497 | 524 |
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| rsGFP (red shifted GFP, S65T) | 498 | 516 |
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| sgGFP™ (super glow GFP) | 472 | 506 |
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| Sodium Green | 507 | 532 |
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| SpectrumGreen | 497 | 538 |
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
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| SYTO 11 | 506 | 525 |
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| SYTO 13 | 448 | 506 |
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| SYTO 16 | 489 | 520 |
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| SYTO 9 | 483 | 500 |
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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| SYTOX Green-DNA | 504 | 524 |
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| TO-PRO-1 | 515 | 531 |
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| TOTO-1 | 514 | 531 |
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| TurboGFP | 482 | 503 |
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| Vybrant DyeCycle Green | 506 | 534 |
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| YO-PRO-1 | 491 | 506 |
|
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| YOYO-1 | 491 | 508 |
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| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-525/45-25
FL-004680 / 0
- Tavg > 93% 502.5 – 547.5 nm
- Center Wavelength of 525 nm and GMBW of 45 nm
- FWHM of 50.6 nm
FF02-482/18-25
FL-004712 / 0
- Tavg > 93% 473 – 491 nm
- Center Wavelength of 482 nm and GMBW of 18 nm
- FWHM of 24.4 nm
Di02-R488-25x36
FL-007135 / 0
- Laser Dichroic Beamsplitter with edge at 495.5 nm
- Rabs > 94% 471 – 491 nm
- Ravg > 90% 350 – 471 nm
- Tavg > 93% 499.8 – 900 nm
- Flatness / RWE: Laser
Custom sizing delivery confirmed ARO (sizing fee applies).