This full-multiband set is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF488/561-A
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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2,102.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 491 nm, 568.2 nm |
|---|---|
| Laser Wavelength 1 (low) | 473 nm |
| Laser Wavelength 2 | 488 nm |
| Laser Wavelength 3 | 561.4 nm |
total selected
Clear selection
Select all 3 products from all pages.
FF01-482/563-25
FL-004303 / 0
- Tavg > 93% 473 – 491 nm & Tavg > 93% 559 – 568 nm
- Center Wavelengths of 482 nm & 563.5 nm and GMBWs of 18 nm & 9 nm
FF01-523/610-25
FL-004544 / 0
- Tavg > 93% 503 – 543 nm & Tavg > 93% 584 – 636 nm
- Center Wavelengths of 523 nm & 610 nm and GMBWs of 40 nm & 52 nm
Di03-R488/561-t1-25x36
FL-007037 / 0
• Laser Dichroic Beamsplitter with edges at 500 nm & 575.5 nm
• Rabs > 94% 471 – 491 nm & Rabs > 94% 559 – 568.2 nm
• Ravg > 90% 350 – 471 nm
• Tavg > 93% 503.3 – 543 nm & Tavg > 93% 582.4 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF
• Rabs > 94% 471 – 491 nm & Rabs > 94% 559 – 568.2 nm
• Ravg > 90% 350 – 471 nm
• Tavg > 93% 503.3 – 543 nm & Tavg > 93% 582.4 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF
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This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
|
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
|
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
|
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
|
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 430 | 431 | 541 |
|
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| Alexa Fluor® 488 | 499 | 520 |
|
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
|
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
|
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
|
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
|
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
|
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| BODIPY TMR-X | 544 | 573 |
|
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
|
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| Calcein | 494 | 514 |
|
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
|
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| Calcium Orange | 589 | 609 |
|
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
|
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| CellTracker Red CMTPX | 586 | 614 |
|
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| CFP2 | 490 | 510 |
|
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| Cy2™ | 492 | 507 |
|
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| Cy3.5™ | 578 | 591 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
|
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| Dendra2 (Red) | 554 | 569 |
|
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
|
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| DyLight 594 | 593 | 618 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
|
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| Ethidium bromide | 518 | 603 |
|
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| Ethidium homodimer | 527 | 617 |
|
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| FITC (Fluorescein) | 495 | 519 |
|
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| HiLyte Fluor™ 594 | 592 | 616 |
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| Hypericin | 600 | 603 |
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| KFP-Red | 575 | 599 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LOLO-1 | 568 | 580 |
|
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
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| mKate2 | 588 | 633 |
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| mPlum | 589 | 649 |
|
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
|
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
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| Orange 552 | 552 | 574 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
|
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Propidium Iodide (PI) | 305 | 617 |
|
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
|
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| Qdot® 625 Nanocrystals | 300 | 621 |
|
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| ratiometric pHluorin pH5 | 500 | 509 |
|
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| ReAsH-CCPGCC | 592 | 606 |
|
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| Red 580 | 580 | 603 |
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| Resorufin | 571 | 584 |
|
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| Rhod-2 | 553 | 577 |
|
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| Rhodamine 110 | 497 | 519 |
|
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
|
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| Rhodamine phalloidin | 558 | 575 |
|
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
|
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumOrange | 554 | 587 |
|
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| TO-PRO-1 | 515 | 531 |
|
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| TOTO-1 | 514 | 531 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboFP635(Katushka) | 591 | 638 |
|
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| TurboGFP | 482 | 503 |
|
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| TurboRFP | 553 | 573 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-1 | 491 | 506 |
|
|
| YOYO-1 | 491 | 508 |
|
|
| YOYO-3 | 611 | 631 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-482/563-25
FL-004303 / 0
- Tavg > 93% 473 – 491 nm & Tavg > 93% 559 – 568 nm
- Center Wavelengths of 482 nm & 563.5 nm and GMBWs of 18 nm & 9 nm