This full-multiband set is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488/561-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,994.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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| Laser Wavelength 1 (high) | 491 nm, 568.2 nm |
|---|---|
| Laser Wavelength 1 (low) | 473 nm |
| Laser Wavelength 2 | 488 nm |
| Laser Wavelength 3 | 561.4 nm |
total selected
Clear selection
Select all 3 products from all pages.
FF01-482/563-25
FL-004303 / 0
- Tavg > 93% 473 – 491 nm & Tavg > 93% 559 – 568 nm
- Center Wavelengths of 482 nm & 563.5 nm and GMBWs of 18 nm & 9 nm
FF01-523/610-25
FL-004544 / 0
- Tavg > 93% 503 – 543 nm & Tavg > 93% 584 – 636 nm
- Center Wavelengths of 523 nm & 610 nm and GMBWs of 40 nm & 52 nm
Di01-R488/561-25x36
FL-006697 / 0
- Laser Dichroic Beamsplitter with edges at 500 nm & 575.5 nm
- Rabs > 94% 471 – 491 nm & Rabs > 94% 559 – 568.2 nm
- Tavg > 93% 503.3 – 543 nm & Tavg > 93% 582.4 – 800 nm
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This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 2-dodecylresorufin-lipid | 582 | 595 |
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| 5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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| 5-FAM (5-carboxyfluorescein) | 492 | 518 |
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| 5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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| 5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
|
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| 7-AAD (7-aminoactinomycin D) | 548 | 648 |
|
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| Acridine Orange (+DNA) | 502 | 526 |
|
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| Alexa Fluor® 430 | 431 | 541 |
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| Alexa Fluor® 488 | 499 | 520 |
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| Alexa Fluor® 500 | 503 | 525 |
|
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| Alexa Fluor® 546 | 556 | 572 |
|
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| Alexa Fluor® 568 | 579 | 603 |
|
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| Alexa Fluor® 594 | 590 | 618 |
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| AmCyan1 | 458 | 489 |
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| Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
|
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| AsRed 2 | 576 | 592 |
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| ATTO 488 | 500 | 525 |
|
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| ATTO 495 | 495 | 527 |
|
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| ATTO 550 | 553 | 576 |
|
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| ATTO 565 | 563 | 592 |
|
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| ATTO 590 | 594 | 624 |
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| BCECF (pH 5.5) | 481 | 518 |
|
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| BCECF (pH 9.0) | 502 | 528 |
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| BOBO™-3 | 570 | 605 |
|
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| BODIPY FL | 505 | 512 |
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| BODIPY FL-MeOH | 502 | 511 |
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| BODIPY TMR-X | 544 | 573 |
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| BODIPY TR-X (MeOH) | 544 | 573 |
|
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| BODIPY TR-X phallacidin | 590 | 621 |
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| Calcein | 494 | 514 |
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| Calcium Crimson | 589 | 609 |
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| Calcium Green-1 | 507 | 529 |
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| Calcium Orange | 589 | 609 |
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| CellTrace BODIPY TR methyl ester | 598 | 625 |
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| CellTracker Red CMTPX | 586 | 614 |
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| CFP2 | 490 | 510 |
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| Cy2™ | 492 | 507 |
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| Cy3.5™ | 578 | 591 |
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| CyQUANT GR-DNA | 502 | 523 |
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| DAF-FM-NO | 495 | 519 |
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| Dendra2 (Green) | 491 | 507 |
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| Dendra2 (Red) | 554 | 569 |
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| DiO | 475 | 500 |
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| Dronpa | 502 | 516 |
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| DsRed | 559 | 583 |
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| DsRed-Express | 556 | 584 |
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| dTomato | 556 | 582 |
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| DY-505-Phalloidin | 503 | 530 |
|
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| DY-590 | 582 | 599 |
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| DyLight 594 | 593 | 618 |
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| ecliptic pHluorin pH5.5 | 473 | 507 |
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| Emerald | 491 | 511 |
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| Ethidium bromide | 518 | 603 |
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| Ethidium homodimer | 527 | 617 |
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| FITC (Fluorescein) | 495 | 519 |
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| FlAsH-CCPFCC | 511 | 530 |
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| Fluo-3 | 506 | 527 |
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| Fluo-4 | 494 | 516 |
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| Fluorescein dextran | 501 | 524 |
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| Fluorescein-pH 8.0 | 489 | 517 |
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| Fluoro-Emerald | 494 | 518 |
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| FluoSpheres Red fluorescent microspheres | 577 | 607 |
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| FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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| GFP (EGFP) | 489 | 511 |
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| Green 496 | 496 | 520 |
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| Green 500 | 501 | 524 |
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| HcRed1 | 588 | 618 |
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| HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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| HCS LipidTOX Green phospholipidosis | 504 | 536 |
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| HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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| HCS LipidTOX Red phospholipidosis | 584 | 608 |
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| HiLyte Fluor™ 488 | 497 | 526 |
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| HiLyte Fluor™ 594 | 592 | 616 |
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| Hypericin | 600 | 603 |
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| KFP-Red | 575 | 599 |
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| LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
|
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| LOLO-1 | 568 | 580 |
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| LysoSensor Green | 448 | 502 |
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| LysoTracker Green | 501 | 509 |
|
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| LysoTracker Red | 573 | 592 |
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| LysoTracker Yellow HCK-123 | 488 | 565 |
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| Magnesium Green | 507 | 531 |
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| Magnesium Orange | 550 | 574 |
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| mApple | 566 | 594 |
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| mCherry | 587 | 610 |
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| Merocyanine 540 | 559 | 579 |
|
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| mHoneyDew | 478 | 562 |
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| MitoTracker™ Green | 490 | 512 |
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| MitoTracker™ Orange | 551 | 575 |
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| MitoTracker™ Red | 578 | 598 |
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| mKate2 | 588 | 633 |
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| mPlum | 589 | 649 |
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| mRFP | 555 | 583 |
|
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| mRFP1 | 584 | 700 |
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| mRuby | 558 | 605 |
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| mStrawberry | 574 | 596 |
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| mTangerine | 568 | 585 |
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| mWasabi | 493 | 509 |
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| NBD-X (MeOH) | 467 | 538 |
|
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| NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
|
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| Nile red-phospolipid | 553 | 637 |
|
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| Orange 552 | 552 | 574 |
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| Oregon Green™ 488 | 498 | 526 |
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| Oregon Green™ 514 | 513 | 532 |
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| pHrodo™, succinimidyl ester | 560 | 587 |
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| PicoGreen® | 502 | 522 |
|
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| PKH67 | 488 | 500 |
|
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| Propidium Iodide (PI) | 305 | 617 |
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| Pro-Q Diamond | 556 | 583 |
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| Qdot® 525 Nanocrystals | 300 | 525 |
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| Qdot® 545 Nanocrystals | 300 | 543 |
|
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| Qdot® 585 Nanocrystals | 300 | 588 |
|
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| Qdot® 605 Nanocrystals | 300 | 602 |
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| Qdot® 625 Nanocrystals | 300 | 621 |
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| ratiometric pHluorin pH5 | 500 | 509 |
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| ReAsH-CCPGCC | 592 | 606 |
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| Red 580 | 580 | 603 |
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| Resorufin | 571 | 584 |
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| Rhod-2 | 553 | 577 |
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| Rhodamine 110 | 497 | 519 |
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| Rhodamine 123 | 507 | 529 |
|
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| Rhodamine Green | 497 | 523 |
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| Rhodamine phalloidin | 558 | 575 |
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| Rhodamine Red-X | 573 | 591 |
|
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| Rhodol Green | 497 | 524 |
|
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| R-phycoerythrin (PE) | 565 | 576 |
|
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| sgGFP™ (super glow GFP) | 472 | 506 |
|
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| SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
|
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| SNARF-1 488nm (ph 6.0) | 548 | 586 |
|
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| SNARF-1 488nm (ph 9.0) | 576 | 638 |
|
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| SNARF-1 514nm (ph 6.0) | 549 | 587 |
|
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| SNARF-1 514nm (ph 9.0) | 576 | 638 |
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| Sodium Green | 507 | 532 |
|
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| SpectrumGreen | 497 | 538 |
|
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| SpectrumOrange | 554 | 587 |
|
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| Sulforhodamine 101-EtOH | 578 | 593 |
|
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| SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
|
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| SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
|
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| SYBR Safe DNA gel stain-DNA | 509 | 526 |
|
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| SYPRO Ruby protein gel stain | 469 | 631 |
|
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| SYTO 11 | 506 | 525 |
|
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| SYTO 13 | 448 | 506 |
|
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| SYTO 16 | 489 | 520 |
|
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| SYTO 9 | 483 | 500 |
|
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| SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
|
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| SYTOX Green-DNA | 504 | 524 |
|
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| Tetramethylrhodamine dextran | 554 | 582 |
|
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| Texas Red dextran | 592 | 613 |
|
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| Texas Red DHPE | 584 | 608 |
|
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| TO-PRO-1 | 515 | 531 |
|
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| TOTO-1 | 514 | 531 |
|
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| TRITC (Tetramethylrhodamine) | 552 | 578 |
|
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| TurboFP635(Katushka) | 591 | 638 |
|
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| TurboGFP | 482 | 503 |
|
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| TurboRFP | 553 | 573 |
|
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| Vybrant DyeCycle Green | 506 | 534 |
|
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| wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
|
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| X-Rhod-1 Indicator | 580 | 601 |
|
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| YO-PRO-1 | 491 | 506 |
|
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| YOYO-1 | 491 | 508 |
|
|
| YOYO-3 | 611 | 631 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-482/563-25
FL-004303 / 0
- Tavg > 93% 473 – 491 nm & Tavg > 93% 559 – 568 nm
- Center Wavelengths of 482 nm & 563.5 nm and GMBWs of 18 nm & 9 nm