This "Pinkel" multiband set includes single-band excitation filters and a dual-band emission & dichroic filter. It is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF or LDMK?
Our previous version is still available for those cube configurations LF488/561-2X-B
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Laser Wavelength 1 (high) | 491 nm, 568.2 nm |
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Laser Wavelength 1 (low) | 473 nm |
Laser Wavelength 2 | 488 nm |
Laser Wavelength 3 | 561.4 nm |
FL-004544 / 0
FL-004686 / 0
FL-004712 / 0
FL-007037 / 0
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This set is mounted in a ZEISS Microscopy FL CUBE EC P&C Fluorescence Filter Holder, which is compatible with Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner, and Axio Scope A1, from ZEISS Microscopy.
Filter Role | Filter Size | Maximum Thickness |
Exciter (Diameter) | 25.0 mm | 5.0 mm |
Emitter (Diameter) | 25.0 mm | 5.0 mm |
Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm |
Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
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2-dodecylresorufin-lipid | 582 | 595 |
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5-carboxy-2,7-dichlorofluorescein | 500 | 525 |
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5-FAM (5-carboxyfluorescein) | 492 | 518 |
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5-ROX (carboxy-X-rhodamine) | 578 | 604 |
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5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) | 542 | 568 |
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7-AAD (7-aminoactinomycin D) | 548 | 648 |
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Acridine Orange (+DNA) | 502 | 526 |
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Alexa Fluor® 430 | 431 | 541 |
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Alexa Fluor® 488 | 499 | 520 |
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Alexa Fluor® 500 | 503 | 525 |
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Alexa Fluor® 546 | 556 | 572 |
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Alexa Fluor® 568 | 579 | 603 |
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Alexa Fluor® 594 | 590 | 618 |
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AmCyan1 | 458 | 489 |
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Amplex UltraRed peroxidation product (pH 7.5) | 568 | 581 |
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AsRed 2 | 576 | 592 |
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ATTO 488 | 500 | 525 |
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ATTO 495 | 495 | 527 |
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ATTO 550 | 553 | 576 |
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ATTO 565 | 563 | 592 |
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ATTO 590 | 594 | 624 |
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BCECF (pH 5.5) | 481 | 518 |
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BCECF (pH 9.0) | 502 | 528 |
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BOBO™-3 | 570 | 605 |
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BODIPY FL | 505 | 512 |
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BODIPY FL-MeOH | 502 | 511 |
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BODIPY TMR-X | 544 | 573 |
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BODIPY TR-X (MeOH) | 544 | 573 |
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BODIPY TR-X phallacidin | 590 | 621 |
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Calcein | 494 | 514 |
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Calcium Crimson | 589 | 609 |
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Calcium Green-1 | 507 | 529 |
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Calcium Orange | 589 | 609 |
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CellTrace BODIPY TR methyl ester | 598 | 625 |
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CellTracker Red CMTPX | 586 | 614 |
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CFP2 | 490 | 510 |
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Cy2™ | 492 | 507 |
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Cy3.5™ | 578 | 591 |
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CyQUANT GR-DNA | 502 | 523 |
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DAF-FM-NO | 495 | 519 |
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Dendra2 (Green) | 491 | 507 |
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Dendra2 (Red) | 554 | 569 |
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DiO | 475 | 500 |
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Dronpa | 502 | 516 |
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DsRed | 559 | 583 |
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DsRed-Express | 556 | 584 |
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dTomato | 556 | 582 |
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DY-505-Phalloidin | 503 | 530 |
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DY-590 | 582 | 599 |
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DyLight 594 | 593 | 618 |
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ecliptic pHluorin pH5.5 | 473 | 507 |
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Emerald | 491 | 511 |
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Ethidium bromide | 518 | 603 |
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Ethidium homodimer | 527 | 617 |
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FITC (Fluorescein) | 495 | 519 |
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FlAsH-CCPFCC | 511 | 530 |
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Fluo-3 | 506 | 527 |
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Fluo-4 | 494 | 516 |
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Fluorescein dextran | 501 | 524 |
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Fluorescein-pH 8.0 | 489 | 517 |
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Fluoro-Emerald | 494 | 518 |
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FluoSpheres Red fluorescent microspheres | 577 | 607 |
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FluoSpheres Yellow-Green fluorescent microspheres | 503 | 514 |
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GFP (EGFP) | 489 | 511 |
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Green 496 | 496 | 520 |
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Green 500 | 501 | 524 |
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HcRed1 | 588 | 618 |
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HCS LipidTOX Green neutral lipid stain | 498 | 507 |
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HCS LipidTOX Green phospholipidosis | 504 | 536 |
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HCS LipidTOX Red neutral lipid stain | 582 | 616 |
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HCS LipidTOX Red phospholipidosis | 584 | 608 |
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HiLyte Fluor™ 488 | 497 | 526 |
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HiLyte Fluor™ 594 | 592 | 616 |
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Hypericin | 600 | 603 |
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KFP-Red | 575 | 599 |
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LIVE-DEAD Fixable Green Dead Cell Stain | 498 | 525 |
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LOLO-1 | 568 | 580 |
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LysoSensor Green | 448 | 502 |
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LysoTracker Green | 501 | 509 |
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LysoTracker Red | 573 | 592 |
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LysoTracker Yellow HCK-123 | 488 | 565 |
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Magnesium Green | 507 | 531 |
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Magnesium Orange | 550 | 574 |
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mApple | 566 | 594 |
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mCherry | 587 | 610 |
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Merocyanine 540 | 559 | 579 |
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mHoneyDew | 478 | 562 |
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MitoTracker™ Green | 490 | 512 |
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MitoTracker™ Orange | 551 | 575 |
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MitoTracker™ Red | 578 | 598 |
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mKate2 | 588 | 633 |
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mPlum | 589 | 649 |
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mRFP | 555 | 583 |
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mRFP1 | 584 | 700 |
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mRuby | 558 | 605 |
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mStrawberry | 574 | 596 |
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mTangerine | 568 | 585 |
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mWasabi | 493 | 509 |
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NBD-X (MeOH) | 467 | 538 |
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NeuroTrace 500/525 Green Fluorescent Nissl Stain | 495 | 524 |
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Nile red-phospolipid | 553 | 637 |
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Orange 552 | 552 | 574 |
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Oregon Green™ 488 | 498 | 526 |
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Oregon Green™ 514 | 513 | 532 |
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pHrodo™, succinimidyl ester | 560 | 587 |
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PicoGreen® | 502 | 522 |
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PKH67 | 488 | 500 |
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Propidium Iodide (PI) | 305 | 617 |
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Pro-Q Diamond | 556 | 583 |
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Qdot® 525 Nanocrystals | 300 | 525 |
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Qdot® 545 Nanocrystals | 300 | 543 |
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Qdot® 585 Nanocrystals | 300 | 588 |
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Qdot® 605 Nanocrystals | 300 | 602 |
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Qdot® 625 Nanocrystals | 300 | 621 |
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ratiometric pHluorin pH5 | 500 | 509 |
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ReAsH-CCPGCC | 592 | 606 |
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Red 580 | 580 | 603 |
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Resorufin | 571 | 584 |
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Rhod-2 | 553 | 577 |
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Rhodamine 110 | 497 | 519 |
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Rhodamine 123 | 507 | 529 |
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Rhodamine Green | 497 | 523 |
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Rhodamine phalloidin | 558 | 575 |
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Rhodamine Red-X | 573 | 591 |
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Rhodol Green | 497 | 524 |
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R-phycoerythrin (PE) | 565 | 576 |
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sgGFP™ (super glow GFP) | 472 | 506 |
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SNARF (carboxy) 514 Excitation pH9 | 576 | 638 |
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SNARF-1 488nm (ph 6.0) | 548 | 586 |
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SNARF-1 488nm (ph 9.0) | 576 | 638 |
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SNARF-1 514nm (ph 6.0) | 549 | 587 |
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SNARF-1 514nm (ph 9.0) | 576 | 638 |
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Sodium Green | 507 | 532 |
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SpectrumGreen | 497 | 538 |
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SpectrumOrange | 554 | 587 |
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Sulforhodamine 101-EtOH | 578 | 593 |
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SYBR Gold nucleic acid gel stain-DNA | 496 | 539 |
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SYBR Green I nucleic acid gel stain-DNA | 498 | 522 |
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SYBR Safe DNA gel stain-DNA | 509 | 526 |
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SYPRO Ruby protein gel stain | 469 | 631 |
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SYTO 11 | 506 | 525 |
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SYTO 13 | 448 | 506 |
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SYTO 16 | 489 | 520 |
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SYTO 9 | 483 | 500 |
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SYTO RNASelect geen fluorescent cell stain | 503 | 527 |
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SYTOX Green-DNA | 504 | 524 |
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Tetramethylrhodamine dextran | 554 | 582 |
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Texas Red dextran | 592 | 613 |
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Texas Red DHPE | 584 | 608 |
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TO-PRO-1 | 515 | 531 |
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TOTO-1 | 514 | 531 |
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TRITC (Tetramethylrhodamine) | 552 | 578 |
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TurboFP635(Katushka) | 591 | 638 |
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TurboGFP | 482 | 503 |
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TurboRFP | 553 | 573 |
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Vybrant DyeCycle Green | 506 | 534 |
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wtGFP (wild type GFP, non-UV excitation) | 474 | 509 |
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X-Rhod-1 Indicator | 580 | 601 |
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YO-PRO-1 | 491 | 506 |
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YOYO-1 | 491 | 508 |
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YOYO-3 | 611 | 631 |
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Topic | Description |
Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |