BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

Semrock Part Number: LF488/561-2X2M-C-NTE

IDEX Part Number: S-001810  /

Semrock

This "Sedat" multiband set includes single-band exciter and emission filters, and a dual-band dichroic. It is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF or LDMK?
Our previous version is still available for those cube configurations LF488/561-2X2M-B

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    US Domestic pricing – contact your local distributor for other currency list prices
  • Price Not Available.
  • 2,607.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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Laser Wavelength 1 (high) 491 nm, 568.2 nm
Laser Wavelength 1 (low) 473 nm
Laser Wavelength 2 488 nm
Laser Wavelength 3 561.4 nm

FF01-525/45-25

FL-004680 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 502.5 – 547.5 nm
  • Center Wavelength of 525 nm and GMBW of 45 nm
  • FWHM of 50.6 nm

FF01-563/9-25

FL-004686 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 559 – 568 nm
  • Center Wavelength of 563.5 nm and GMBW of 9 nm
  • FWHM of 14.5 nm

FF01-609/54-25

FL-004693 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 582 – 636 nm
  • Center Wavelength of 609 nm and GMBW of 54 nm
  • FWHM of 60.6 nm

FF02-482/18-25

FL-004712 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 473 – 491 nm
  • Center Wavelength of 482 nm and GMBW of 18 nm
  • FWHM of 24.4 nm

Di03-R488/561-t1-25x36

FL-007037 / 0

Out of Stock Backordered In Stock
• Laser Dichroic Beamsplitter with edges at 500 nm & 575.5 nm
• Rabs > 94% 471 – 491 nm & Rabs > 94% 559 – 568.2 nm
• Ravg > 90% 350 – 471 nm
• Tavg > 93% 503.3 – 543 nm & Tavg > 93% 582.4 – 1200 nm
• Flatness / RWE: Super-resolution / TIRF

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This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm3.5 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.1 mm
(Can accept 2 mm dichroic)
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
2-dodecylresorufin-lipid 582 595
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
5-ROX (carboxy-X-rhodamine) 578 604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0) 542 568
6-carboxyrhodamine 6G 525 555
7-AAD (7-aminoactinomycin D) 548 648
Acridine Orange (+DNA) 502 526
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
Alexa Fluor® 546 556 572
Alexa Fluor® 568 579 603
Alexa Fluor® 594 590 618
AmCyan1 458 489
Amplex UltraRed peroxidation product (pH 7.5) 568 581
AsRed 2 576 592
ATTO 488 500 525
ATTO 495 495 527
ATTO 550 553 576
ATTO 565 563 592
ATTO 590 594 624
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BOBO™-3 570 605
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
BODIPY TMR-X 544 573
BODIPY TR-X (MeOH) 544 573
BODIPY TR-X phallacidin 590 621
Calcein 494 514
Calcium Crimson 589 609
Calcium Green-1 507 529
Calcium Orange 589 609
CellTrace BODIPY TR methyl ester 598 625
CellTracker Red CMTPX 586 614
CFP2 490 510
Cy2™ 492 507
Cy3.5™ 578 591
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
Dendra2 (Red) 554 569
DiA 457 586
DiO 475 500
Dronpa 502 516
DsRed 559 583
DsRed-Express 556 584
dTomato 556 582
DY-505-Phalloidin 503 530
DY-590 582 599
DyLight 594 593 618
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
Ethidium bromide 518 603
Ethidium homodimer 527 617
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Red fluorescent microspheres 577 607
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HcRed1 588 618
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HCS LipidTOX Red neutral lipid stain 582 616
HCS LipidTOX Red phospholipidosis 584 608
HiLyte Fluor™ 488 497 526
HiLyte Fluor™ 594 592 616
Hypericin 600 603
KFP-Red 575 599
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LOLO-1 568 580
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Red 573 592
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
Magnesium Orange 550 574
mApple 566 594
mCherry 587 610
Merocyanine 540 559 579
mHoneyDew 478 562
MitoTracker™ Green 490 512
MitoTracker™ Orange 551 575
MitoTracker™ Red 578 598
mKate2 588 633
mPlum 589 649
mRFP 555 583
mRFP1 584 700
mRuby 558 605
mStrawberry 574 596
mTangerine 568 585
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Nile red-phospolipid 553 637
Orange 552 552 574
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
pHrodo™, succinimidyl ester 560 587
PicoGreen® 502 522
PKH67 488 500
Propidium Iodide (PI) 305 617
Pro-Q Diamond 556 583
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
ratiometric pHluorin pH5 500 509
ReAsH-CCPGCC 592 606
Red 580 580 603
Resorufin 571 584
Rhod-2 553 577