This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from CFP and other similar fluorophores while offering superb blocking of popular 442 nm (solid-state) lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, or LDMK?
Our previous version is still available for those cube configurations LF442-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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1,330.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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0.000.00 -
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- Notify me when available
We now offer LFOV (large field of view) cube sets compatible with Nikon Eclipse Ti2 microscopes. Please contact us for more information.
Set Components
Di03-R442-t1-25x36FL-006967
Each
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FL-006967 |
|
FF01-448/20-25FL-004533
Each
|
FL-004533 |
|
FF01-482/25-25FL-004256
Each
|
FL-004256 |
total selected
Clear selection
Select all 2 products from all pages.
LF442-C-NTE
S-000457 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF442-C-ZHE
S-000891 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
|
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| AmCyan1 | 458 | 489 |
|
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| Aqua 431 | 430 | 478 |
|
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| ATTO 425 | 436 | 484 |
|
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| ATTO 465 | 453 | 507 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BOBO™-1 | 461 | 484 |
|
|
| BV480 - BD Horizon Brilliant™ Violet 480 | 440 | 479 |
|
|
| Cerulean | 434 | 473 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| DEAC | 432 | 472 |
|
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| ECFP | 435 | 475 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| LysoSensor Green | 448 | 502 |
|
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| mTFP1 | 462 | 492 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| SpectrumAqua | 434 | 481 |
|
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| SYTO 45 | 451 | 485 |
|
|
| SYTOX Blue | 444 | 470 |
|
|
| TagCFP | 458 | 480 |
|
total selected
Clear selection
Select all 2 products from all pages.
LF442-C-NTE
S-000457 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
LF442-C-ZHE
S-000891 / 0
This set is delivered mounted in microscope filter holder, often called 'cube', ready for your microscope.
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
1 of 1
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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