This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from CFP and other similar fluorophores while offering superb blocking of popular 442 nm (solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.
Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF442-C
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0.00 USD EachUS Domestic pricing – contact your local distributor for other currency list prices
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| Laser Wavelength 1 (high) | 457.9 nm |
|---|---|
| Laser Wavelength 1 (low) | 441.6 nm |
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FF01-482/25-25
FL-004256 / 0
- Tavg > 93% 469.5 – 494.5 nm
- Center Wavelength of 482 nm and GMBW of 25 nm
- FWHM of 30.1 nm
FF01-448/20-25
FL-004533 / 0
- Tavg > 93% 438 – 458 nm
- Center Wavelength of 448 nm and GMBW of 20 nm
- FWHM of 25 nm
Di02-R442-25x36
FL-007007 / 0
- Laser Dichroic Beamsplitter with edge at 462 nm
- Rabs > 94% 439 – 457.9 nm
- Ravg > 90% 350 – 439 nm
- Tavg > 93% 466.1 – 900 nm
- Flatness / RWE: Laser
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| Acridine Yellow | 470 | 550 |
|
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| AmCyan1 | 458 | 489 |
|
|
| Aqua 431 | 430 | 478 |
|
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| ATTO 425 | 436 | 484 |
|
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| ATTO 465 | 453 | 507 |
|
|
| BD Horizon V500 | 410 | 500 |
|
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| BOBO™-1 | 461 | 484 |
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| Cerulean | 434 | 473 |
|
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| CFP (ECFP) | 433 | 475 |
|
|
| DEAC | 432 | 472 |
|
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| ECFP | 435 | 475 |
|
|
| evoglow-Bs1 | 449 | 496 |
|
|
| LysoSensor Green | 448 | 502 |
|
|
| mTFP1 | 462 | 492 |
|
|
| POPO-1 | 433 | 457 |
|
|
| PO-PRO-1 | 433 | 456 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SYTO 45 | 451 | 485 |
|
|
| SYTOX Blue | 444 | 470 |
|
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| TagCFP | 458 | 480 |
|
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
total selected
Clear selection
Select all 3 products from all pages.
FF01-482/25-25
FL-004256 / 0
- Tavg > 93% 469.5 – 494.5 nm
- Center Wavelength of 482 nm and GMBW of 25 nm
- FWHM of 30.1 nm
FF01-448/20-25
FL-004533 / 0
- Tavg > 93% 438 – 458 nm
- Center Wavelength of 448 nm and GMBW of 20 nm
- FWHM of 25 nm
Di02-R442-25x36
FL-007007 / 0
- Laser Dichroic Beamsplitter with edge at 462 nm
- Rabs > 94% 439 – 457.9 nm
- Ravg > 90% 350 – 439 nm
- Tavg > 93% 466.1 – 900 nm
- Flatness / RWE: Laser
1 of 1
This set is mounted in an Evident Scientific (Olympus) OFF Fluorescence Filter Holder, which is compatible with the BX3 (BX53, BX63) and IX3 Series (IX53, IX73, IX83) of Evident Scientific (Olympus) microscopes.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 5.0 mm |
| Dichroic (L × W) | 26 mm x 38 mm | 1.5 mm |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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