BrightLine® single-band laser filter set, optimized for 442 nm laser sources

Semrock Part Number: LF442-B-LDMK

IDEX Part Number: S-001771  /

Semrock

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from CFP and other similar fluorophores while offering superb blocking of popular 442 nm (solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF442-C

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  • 1,669.00 USD Each
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Laser Wavelength 1 (high) 457.9 nm
Laser Wavelength 1 (low) 441.6 nm

FF01-482/25-25

FL-004256 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 469.5 – 494.5 nm
  • Center Wavelength of 482 nm and GMBW of 25 nm
  • FWHM of 30.1 nm

FF01-448/20-25

FL-004533 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 438 – 458 nm
  • Center Wavelength of 448 nm and GMBW of 20 nm
  • FWHM of 25 nm

Di02-R442-25x36

FL-007007 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 462 nm
  • Rabs > 94% 439 – 457.9 nm
  • Ravg > 90% 350 – 439 nm
  • Tavg > 93% 466.1 – 900 nm
  • Flatness / RWE: Laser

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
Acridine Yellow 470 550
AmCyan1 458 489
Aqua 431 430 478
ATTO 425 436 484
ATTO 465 453 507
BD Horizon V500 410 500
BOBO™-1 461 484
Cerulean 434 473
CFP (ECFP) 433 475
DEAC 432 472
ECFP 435 475
evoglow-Bs1 449 496
LysoSensor Green 448 502
mTFP1 462 492
POPO-1 433 457
PO-PRO-1 433 456
SpectrumAqua 434 481
SYTO 45 451 485
SYTOX Blue 444 470
TagCFP 458 480
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.

Specifications

FF01-482/25-25

FL-004256 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 469.5 – 494.5 nm
  • Center Wavelength of 482 nm and GMBW of 25 nm
  • FWHM of 30.1 nm

FF01-448/20-25

FL-004533 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 438 – 458 nm
  • Center Wavelength of 448 nm and GMBW of 20 nm
  • FWHM of 25 nm

Di02-R442-25x36

FL-007007 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 462 nm
  • Rabs > 94% 439 – 457.9 nm
  • Ravg > 90% 350 – 439 nm
  • Tavg > 93% 466.1 – 900 nm
  • Flatness / RWE: Laser

1 of 1

This set is mounted in a Leica Microsystems DM-K (“Large”) Fluorescence Filter Holder, which is compatible with Leica microscopes DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)21.8 mm5.0 mm
Emitter (Diameter)23.3 mm5.0 mm
Dichroic (L × W)22.0 mm × 29.0 mm1.1 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.