BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 405 nm laser sources

Semrock Part Number: LF405/LP-C-OMF

IDEX Part Number: S-000449  /

Semrock

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/LP-B

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  • Price Not Available.
  • 1,872.00 USD Each
    US Domestic pricing – contact your local distributor for other currency list prices
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FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

Di03-R405-t1-25x36

FL-007078 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 414 nm,
  • Rabs > 94% 372 – 410 nm
  • Ravg > 90% 350 – 372 nm
  • Tavg > 93% 417.4 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

BLP01-405R-25

FL-008529 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 418 nm
  • 595 cm-1 transition
  • Tavg > 93% from 421.5 – 900 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
1,8-ANS 372 477
4-methylumbelliferone 357 441
7-hydroxy-4-methylcoumarin 360 442
Alexa Fluor® 350 343 441
Alexa Fluor® 405 401 422
Alexa Fluor® 430 431 541
AMCA (Aminomethylcoumarin) 350 448
Aqua 431 430 478
ATTO 390 392 477
ATTO 425 436 484
BD Horizon V450 406 449
BD Horizon V500 410 500
BFP (EBFP) 380 440
BV421 - BD Horizon Brilliant™ Violet 421 407 421
Calcofluor White 351 444
Cascade Blue™ 401 419
Cascade Yellow™ 399 549
CellTrace calcein violet 400 452
CellTracker Violet BMQC+GSH 406 526
CFP (ECFP) 433 475
ChlorophyllA 480 650
DAPI 359 461
Dapoxyl 373 579
DEAC 432 472
DiFMU-pH 9.0 358 450
DY-415 418 470
DyLight 350 351 434
DyLight 405 398 420
ER-Tracker™ Blue-White DPX 372 557
Fura Red™ (high pH) 572 657
Fura-2 (calcium free) 366 515
GFPuv 395 509
HiLyte Fluor™ 405 403 428
Hoechst 33258 352 455
Hoechst 33342 352 455
Hoechst 34580 392 440
LIVE-DEAD Fixable Aqua Dead Cell Stain 367 526
LIVE-DEAD Fixable Violet Dead Cell Stain 403 455
Lucifer yellow 428 544
LysoSensor Blue 374 424
LysoTracker Blue 373 422
Marina Blue 363 461
mBBr+GSH 394 490
Pacific Blue™ 404 455
Pacific Orange 400 551
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
Qdot® 565 Nanocrystals 300 564
Qdot® 585 Nanocrystals 300 588
Qdot® 605 Nanocrystals 300 602
Qdot® 625 Nanocrystals 300 621
Qdot® 655 Nanocrystals 300 654
Qdot® 705 Nanocrystals 300 702
Qdot® 800 Nanocrystals 300 792
sgBFP™ 387 451
Sirius 355 424
SpectrumAqua 434 481
SpectrumBlue 405 449
SYPRO Ruby protein gel stain 469 631
TagBFP 402 457
ThiolTracker Violet+GSH 404 526
Vybrant DyeCycle Violet 369 437

Related Products

FF01-390/40-25

FL-004666 / 0

Out of Stock Backordered In Stock
  • Tavg > 93% 370 – 410 nm
  • Center Wavelength of 390 nm and GMBW of 40 nm
  • FWHM of 44.6 nm

Di03-R405-t1-25x36

FL-007078 / 0

Out of Stock Backordered In Stock
  • Laser Dichroic Beamsplitter with edge at 414 nm,
  • Rabs > 94% 372 – 410 nm
  • Ravg > 90% 350 – 372 nm
  • Tavg > 93% 417.4 – 1200 nm
  •  Flatness / RWE: Super-resolution / TIRF

BLP01-405R-25

FL-008529 / 0

Out of Stock Backordered In Stock
  • Filter with 50% edge at 418 nm
  • 595 cm-1 transition
  • Tavg > 93% from 421.5 – 900 nm

1 of 1

This set is mounted in an Evident Scientific (Olympus) OMF Fluorescence Filter Holder, which is compatible with the AX (AX70), BX2 (BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI), and IX2 Series (IX50, IX51, IX70, IX71, IX81) of Evident Scientific (Olympus) microscopes.

Filter Role Filter Size Maximum Thickness
Exciter (Diameter)25.0 mm5.0 mm
Emitter (Diameter)25.0 mm5.0 mm
Dichroic (L × W)25.2 mm × 35.6 mm1.5 mm
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Working with Optical Density Tables to describe the relationship between OD and transmission with tips for quickly calculating OD
What is Pixel Shift, and how does the Semrock ZERO option help? Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets.