This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.
Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.
Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/LP-B
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EachUS Domestic pricing – contact your local distributor for other currency list prices
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FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
Di03-R405-t1-25x36
FL-007078 / 0
- Laser Dichroic Beamsplitter with edge at 414 nm,
- Rabs > 94% 372 – 410 nm
- Ravg > 90% 350 – 372 nm
- Tavg > 93% 417.4 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
BLP01-405R-25
FL-008529 / 0
- Filter with 50% edge at 418 nm
- 595 cm-1 transition
- Tavg > 93% from 421.5 – 900 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
| Fluorophore | Peak Excitation Wavelength(nm) | Peak Emission Wavelength(nm) | Compatibility | Display on Plot above |
|---|---|---|---|---|
| 1,8-ANS | 372 | 477 |
|
|
| 4-methylumbelliferone | 357 | 441 |
|
|
| 7-hydroxy-4-methylcoumarin | 360 | 442 |
|
|
| Alexa Fluor® 350 | 343 | 441 |
|
|
| Alexa Fluor® 405 | 401 | 422 |
|
|
| Alexa Fluor® 430 | 431 | 541 |
|
|
| AMCA (Aminomethylcoumarin) | 350 | 448 |
|
|
| Aqua 431 | 430 | 478 |
|
|
| ATTO 390 | 392 | 477 |
|
|
| ATTO 425 | 436 | 484 |
|
|
| BD Horizon V450 | 406 | 449 |
|
|
| BD Horizon V500 | 410 | 500 |
|
|
| BFP (EBFP) | 380 | 440 |
|
|
| BV421 - BD Horizon Brilliant™ Violet 421 | 407 | 421 |
|
|
| Calcofluor White | 351 | 444 |
|
|
| Cascade Blue™ | 401 | 419 |
|
|
| Cascade Yellow™ | 399 | 549 |
|
|
| CellTrace calcein violet | 400 | 452 |
|
|
| CellTracker Violet BMQC+GSH | 406 | 526 |
|
|
| CFP (ECFP) | 433 | 475 |
|
|
| ChlorophyllA | 480 | 650 |
|
|
| DAPI | 359 | 461 |
|
|
| Dapoxyl | 373 | 579 |
|
|
| DEAC | 432 | 472 |
|
|
| DiFMU-pH 9.0 | 358 | 450 |
|
|
| DY-415 | 418 | 470 |
|
|
| DyLight 350 | 351 | 434 |
|
|
| DyLight 405 | 398 | 420 |
|
|
| ER-Tracker™ Blue-White DPX | 372 | 557 |
|
|
| Fura Red™ (high pH) | 572 | 657 |
|
|
| Fura-2 (calcium free) | 366 | 515 |
|
|
| GFPuv | 395 | 509 |
|
|
| HiLyte Fluor™ 405 | 403 | 428 |
|
|
| Hoechst 33258 | 352 | 455 |
|
|
| Hoechst 33342 | 352 | 455 |
|
|
| Hoechst 34580 | 392 | 440 |
|
|
| LIVE-DEAD Fixable Aqua Dead Cell Stain | 367 | 526 |
|
|
| LIVE-DEAD Fixable Violet Dead Cell Stain | 403 | 455 |
|
|
| Lucifer yellow | 428 | 544 |
|
|
| LysoSensor Blue | 374 | 424 |
|
|
| LysoTracker Blue | 373 | 422 |
|
|
| Marina Blue | 363 | 461 |
|
|
| mBBr+GSH | 394 | 490 |
|
|
| Pacific Blue™ | 404 | 455 |
|
|
| Pacific Orange | 400 | 551 |
|
|
| Qdot® 525 Nanocrystals | 300 | 525 |
|
|
| Qdot® 545 Nanocrystals | 300 | 543 |
|
|
| Qdot® 565 Nanocrystals | 300 | 564 |
|
|
| Qdot® 585 Nanocrystals | 300 | 588 |
|
|
| Qdot® 605 Nanocrystals | 300 | 602 |
|
|
| Qdot® 625 Nanocrystals | 300 | 621 |
|
|
| Qdot® 655 Nanocrystals | 300 | 654 |
|
|
| Qdot® 705 Nanocrystals | 300 | 702 |
|
|
| Qdot® 800 Nanocrystals | 300 | 792 |
|
|
| sgBFP™ | 387 | 451 |
|
|
| Sirius | 355 | 424 |
|
|
| SpectrumAqua | 434 | 481 |
|
|
| SpectrumBlue | 405 | 449 |
|
|
| SYPRO Ruby protein gel stain | 469 | 631 |
|
|
| TagBFP | 402 | 457 |
|
|
| ThiolTracker Violet+GSH | 404 | 526 |
|
|
| Vybrant DyeCycle Violet | 369 | 437 |
|
total selected
Clear selection
Select all 3 products from all pages.
FF01-390/40-25
FL-004666 / 0
- Tavg > 93% 370 – 410 nm
- Center Wavelength of 390 nm and GMBW of 40 nm
- FWHM of 44.6 nm
Di03-R405-t1-25x36
FL-007078 / 0
- Laser Dichroic Beamsplitter with edge at 414 nm,
- Rabs > 94% 372 – 410 nm
- Ravg > 90% 350 – 372 nm
- Tavg > 93% 417.4 – 1200 nm
- Flatness / RWE: Super-resolution / TIRF
BLP01-405R-25
FL-008529 / 0
- Filter with 50% edge at 418 nm
- 595 cm-1 transition
- Tavg > 93% from 421.5 – 900 nm
1 of 1
This set is mounted in a Nikon NTE Fluorescence Filter Holder, which is compatible with Nikon microscopes TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator.
| Filter Role | Filter Size | Maximum Thickness |
| Exciter (Diameter) | 25.0 mm | 5.0 mm |
| Emitter (Diameter) | 25.0 mm | 3.5 mm |
| Dichroic (L × W) | 25.2 mm × 35.6 mm | 1.1 mm (Can accept 2 mm dichroic) |
| Topic | Description |
| Cleaning Optical Filters | Instructions for cleaning hard coated optical filters |
| Filter Reliability | Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters |
| Working with Optical Density | Tables to describe the relationship between OD and transmission with tips for quickly calculating OD |
| What is Pixel Shift, and how does the Semrock ZERO™ option help? | Why Pixel Shift degrades multicolor fluorescence imaging performance, and how the ZERO™ option mitigates this for Avant and BrightLine filter sets. |
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LPD02-785RU-25
FL-008049